Hynes R O
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3170-4. doi: 10.1073/pnas.70.11.3170.
Putative cell-surface proteins of tissue-culture cells were identified by lactoperoxidase-catalyzed iodination, a technique that attaches label only to proteins outside the cell membrane. Evidence is presented that these proteins are cell derived, not contaminating serum proteins. On "normal" cells, which exhibit density dependence of growth, one protein of high molecular weight was particularly readily iodinated. This protein was easily removed by mild proteolytic digestion, and, in virus-transformed cells, was either absent or unavailable for iodination. The possible relevance of these observations to the control of growth in cell culture is discussed.
通过乳过氧化物酶催化碘化法鉴定了组织培养细胞的推定细胞表面蛋白，该技术仅将标记物附着于细胞膜外的蛋白质上。有证据表明这些蛋白质是细胞来源的，而非污染的血清蛋白。在呈现生长密度依赖性的“正常”细胞上，一种高分子量蛋白质特别容易被碘化。这种蛋白质很容易通过温和的蛋白水解消化去除，并且在病毒转化的细胞中，要么不存在，要么无法进行碘化。讨论了这些观察结果与细胞培养中生长控制的可能相关性。
