Bakst M R, Sexton T J
J Reprod Fertil. 1979 Jan;55(1):1-7. doi: 10.1530/jrf.0.0550001.
The fertilizing capacity, motility and ultrastructure of fowl and turkey spermatozoa were examined at various stages of the freezing process. For both species, fertility and motility were depressed after equilibration with dimethyl-sulphoxide at 5 degrees C. After freezing, motility was maintained at 55% for fowl spermatozoa and 40% for turkey spermatozoa; however, fertility was 55% for the fowl and 0% for the turkey. Qualitatively, the damage to the spermatozoa of both species was nearly identical, as revealed by scanning and transmission electron microscopy. The plasmalemma was the primary site of damage. 'Bent' spermatozoa, coiled tails and swollen mitochondria were also present. Damage to the acrosome was only observed in spermatozoa which had been frozen to -180 degrees or -196 degrees C. These changes were attributed to adverse osmotic conditions. Binding of cationic ferritin to the plasmalemma of spermatozoa from both species remained unaltered.
在冷冻过程的不同阶段，对鸡和火鸡精子的受精能力、活力及超微结构进行了检测。对于这两个物种，在5℃用二甲基亚砜平衡后，受精能力和活力均下降。冷冻后，鸡精子的活力维持在55%，火鸡精子的活力维持在40%；然而，鸡的受精率为55%，火鸡的受精率为0%。定性地说，扫描电镜和透射电镜显示，两个物种精子的损伤几乎相同。质膜是主要的损伤部位。还出现了“弯曲”的精子、卷曲的尾巴和肿胀的线粒体。仅在冷冻至-180℃或-196℃的精子中观察到顶体的损伤。这些变化归因于不利的渗透条件。阳离子铁蛋白与两个物种精子质膜的结合保持不变。
