Bruce C, Taylor W H, Westwood A
Ann Clin Biochem. 1979 Sep;16(5):259-64. doi: 10.1177/000456327901600166.
A radioenzymatic method suitable for the assay of histamine in human blood, urine, plasma, and gastric juice is described. It differs from earlier methods by use of a histamine methyltransferase preparation from pig brain, of high activity tritiated S-adenosylmethionine, and of a heat precipitation step to reduce the previously noted interference from plasma constituents. The method is simpler than those requiring solvent extraction and concentration of histamine, gives recoveries in the range 80-120%, and so sliminates the need for internal standardisation. The method is sensitive and precise with coefficients of variation for blood, urine, and plasma of 5%, 6%, and 13% respectively. The mean +/- standard deviation for normal human plasma histamine is 5 +/- 4 nmol/l, for whole blood 559 +/- 193 nmol/l, and for urine 229 +/- 128 nmol/24h.
本文描述了一种适用于检测人血液、尿液、血浆和胃液中组胺的放射酶法。该方法与早期方法的不同之处在于，它使用了来自猪脑的组胺甲基转移酶制剂、高活性的氚标记S-腺苷甲硫氨酸，以及一个热沉淀步骤，以减少先前发现的血浆成分的干扰。该方法比那些需要对组胺进行溶剂萃取和浓缩的方法更简单，回收率在80%-120%之间，因此无需进行内标法。该方法灵敏且精确，血液、尿液和血浆的变异系数分别为5%、6%和13%。正常人血浆组胺的平均值±标准差为5±4 nmol/l，全血为559±193 nmol/l，尿液为229±128 nmol/24h。
