Navert H, Bérubé R, Wollin A
Can J Physiol Pharmacol. 1985 Jun;63(6):766-72. doi: 10.1139/y85-127.
A method using capillary gas chromatography is described for the determination of histamine and eight of its basic and acid metabolites in a single biological sample of serum, urine, or gastric juice. Ion-exchange chromatography and extraction with organic solvents are used for isolation and purification, and gas chromatography for identification and quantitation. The heptafluorobutyryl derivatives of histamine and some basic metabolites are compatible with nitrogen-phosphorus and electron capture detection modes and offer an excellent sensitivity (detection limit 0.1 pmol with electron capture). The acid metabolites are quantitated after esterification. The linearity range, the sensitivity, a partial study of reproducibility and typical chromatograms show that the method is adaptable to a variety of applications.
本文描述了一种使用毛细管气相色谱法测定血清、尿液或胃液单一生物样本中组胺及其八种碱性和酸性代谢物的方法。采用离子交换色谱法和有机溶剂萃取法进行分离纯化，并用气相色谱法进行鉴定和定量。组胺及其一些碱性代谢物的七氟丁酰衍生物与氮磷检测和电子捕获检测模式兼容，并具有出色的灵敏度（电子捕获检测限为0.1皮摩尔）。酸性代谢物在酯化后进行定量。线性范围、灵敏度、重现性的部分研究以及典型色谱图表明该方法适用于多种应用。
