Rodgers B M, McDonald A P, Talbert J L, Donnelly W H
J Thorac Cardiovasc Surg. 1979 Apr;77(4):543-9.
With advances in equipment design, cryotherapy has been applied increasingly in the management of various integumentary disorders. Recently, endobronchial cryotherapy has been utilized in the treatment of acquired tracheal strictures. To evaluate the effects of cryotherapy upon the esophagus, we cooled the distal esophagus of 12 cats to -70 degrees to -80 degrees C. for 2 minutes with a specially constructed cryoprobe. The animals were put to death in pairs 90 minutes, 4 days, 10 days, 14 days, 28 days, and 42 days following cryotherapy. Immediately prior to sacrifice, all animals were studied with esophageal cinefluoroscopy and esophageal manometrics. All animals tolerated this degree of cryotherapy and gained weight on an ad libitum diet. Manometric studies demonstrated diminished amplitude of peristalsis immediately following cryotherapy, but prompt return to a normal manometric pattern. There was no evidence of esophageal dysfunction on esophageal cinefluoroscopy. Morphologic examination demonstrated superficial ulceration between 4 and 10 days following cryotherapy, with subsequent healing. The esophagus in animals put to death 28 and 42 days following cryotherapy appeared histologically normal. These studies demonstrate the safety of cryotherapy to -70 to -80 degrees C. upon the esophageal wall and suggest investigation of the usefulness of this modality in the treatment of local esophageal webs and strictures.
随着设备设计的进步，冷冻疗法在各种皮肤疾病的治疗中应用越来越广泛。最近，支气管内冷冻疗法已被用于治疗后天性气管狭窄。为了评估冷冻疗法对食管的影响，我们用特制的冷冻探头将12只猫的食管远端冷却至-70℃至-80℃，持续2分钟。在冷冻疗法后的90分钟、4天、10天、14天、28天和42天，将动物成对处死。在处死前，对所有动物进行食管荧光电影摄影和食管测压研究。所有动物都耐受这种程度的冷冻疗法，并且在随意饮食的情况下体重增加。测压研究表明，冷冻疗法后立即出现蠕动幅度减小，但迅速恢复到正常的测压模式。食管荧光电影摄影未发现食管功能障碍的证据。形态学检查显示，冷冻疗法后4至10天出现浅表溃疡，随后愈合。在冷冻疗法后28天和42天处死的动物的食管在组织学上看起来正常。这些研究证明了将食管壁冷冻至-70℃至-80℃的冷冻疗法的安全性，并建议研究这种方法在治疗局部食管蹼和狭窄方面的有效性。
