蜡样芽孢杆菌孢子和营养细胞的蛋白质合成系统。

Protein synthesizing systems from spores and vegetative cells of Bacillus cereus.

作者信息

Douthit H A, Kieras R A, Preston R A

出版信息

Arch Microbiol. 1979 Jun;121(3):235-40. doi: 10.1007/BF00425061.

Abstract

A system of polyphenylalanine synthesis was optimized for a comparison of the polymerizing activities of ribosomes from spores and vegetative cells of Bacillus cereus T. Ribosomes of both types react similarly, showing a magnesium optimum of about 6mM and spermidine optima of about 5mM and 4mM for vegative and spore ribosomes, respectively. These lead to optimum mono- to multivalent cation rations of 9 and 10 respectively at 100 mM ammonium ion. A comparison of the response of these ribosomes to suboptimal concentrations of magnesium and spermidine show that they differ qualitatively from each other, suggesting that they possess different structure, macromolecular or ionic components.

摘要

为比较蜡样芽孢杆菌T孢子和营养细胞核糖体的聚合活性，对聚苯丙氨酸合成系统进行了优化。两种类型的核糖体反应相似，镁的最适浓度约为6mM，营养细胞核糖体和孢子核糖体的亚精胺最适浓度分别约为5mM和4mM。在100mM铵离子存在下，这些分别导致单价至多价阳离子的最佳比例为9和10。对这些核糖体对镁和亚精胺亚最佳浓度的反应进行比较表明，它们在质量上彼此不同，这表明它们具有不同的结构、大分子或离子成分。