用流产布鲁氏菌19号菌株对两只绵羊进行接种后，对其血清中19S和7S免疫球蛋白发育情况的初步研究。
A preliminary study of the development of 19S and 7S immunoglobulins in sera of two sheep vaccinated with Brucella abortus, strain 19.
作者信息
Merriman M J, Rice C E
出版信息
Can J Comp Med. 1969 Jan;33(1):64-7.
The pattern of antibody response to vaccination with Brucella abortus, strain 19, was studied in two sheep. Agglutinative activity was detected by the third and fifth days and complement - fixing activity by the fifth and seventh days post-vaccination. Density gradient centrifugation and DEAE cellulose column chromatography showed the 19S antibody developed first, followed soon after by 7S antibody. The former had disappeared by the 25th day but the latter persisted longer in both sheep. A small amount of 19S antibody was detected in sheep 1 following a booster dose of vaccine but 7S antibody constituted the major secondary response. The standard tube agglutination test was found to be more efficient than the complement-fixation test for titration of 19S antibody. An increase in the salt concentration to 10% in tube agglutination test rendered it more sensitive in demonstrating 7S antibody.
对两只绵羊接种流产布鲁氏菌19号菌株疫苗后的抗体反应模式进行了研究。接种后第三天和第五天检测到凝集活性，接种后第五天和第七天检测到补体结合活性。密度梯度离心和DEAE纤维素柱层析表明，首先产生19S抗体，随后不久产生7S抗体。前者在第25天时消失，但在两只绵羊中后者持续时间更长。在绵羊1接种加强剂量疫苗后检测到少量19S抗体，但7S抗体构成主要的二次反应。发现标准试管凝集试验在滴定19S抗体方面比补体结合试验更有效。在试管凝集试验中将盐浓度提高到10%可使其在检测7S抗体时更敏感。
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