免疫兔抗血清的7S和19S成分与人A组及AB型红细胞的反应
REACTION OF 7S AND 19S COMPONENTS OF IMMUNE RABBIT ANTISERA WITH HUMAN GROUP A AND AB RED CELLS.
作者信息
GREENBURY C L, MOORE D H, NUNN L A
出版信息
Immunology. 1963 Sep;6(5):421-33.
7S and 19S components of rabbit anti-A antibody, labelled with I have been separated by chromatography on DEAE-cellulose. The reactions of these components with human group A, A and AB red cells have been investigated. At saturation point A cells combine with 0.22 μg. of 7S antibody per million cells, this corresponds to a minimum of 8.3×10 A-antigen sites per cell. A cells can take up only one quarter as much antibody, and combine much less avidly with it than do A cells. Cells can take up only about one-fifth as many 19S as 7S antibody molecules. 19S antibody is more avid and, on a molecular basis, is 750 times more efficient at agglutinating red cells than 7S antibody. The significance of these findings is discussed with regard to the differences between A and A antigens, and the valency and mode of attachment to the red cells of the antibodies.
用碘标记的兔抗A抗体的7S和19S成分已通过DEAE - 纤维素柱层析分离。研究了这些成分与人A、A和AB血型红细胞的反应。在饱和点时，A细胞每百万细胞与0.22μg的7S抗体结合，这相当于每个细胞至少有8.3×10个A抗原位点。A细胞只能摄取四分之一的抗体量，并且与抗体的结合亲和力远低于A细胞。细胞摄取的19S抗体分子数量仅约为7S抗体分子的五分之一。19S抗体的亲和力更高，从分子角度来看，其凝集红细胞的效率比7S抗体高750倍。针对A和A抗原之间的差异以及抗体与红细胞结合的价数和方式，对这些发现的意义进行了讨论。
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