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人类血小板蛋白酶活性研究。

Studies on human platelet protease activity.

作者信息

Nachman R L, Ferris B

出版信息

J Clin Invest. 1969 Nov;47(11):2530-40. doi: 10.1172/JCI105935.

DOI:10.1172/JCI105935
PMID:4237782
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC297418/
Abstract

SOLUBILIZED PROTEIN DERIVED FROM HUMAN PLATELETS WAS FRACTIONATED BY DEAE CELLULOSE COLUMN CHROMATOGRAPHY AND ANALYZED FOR PROTEASE ACTIVITY USING THREE SUBSTRATES

denatured bovine hemoglobin, alpha casein, and purified plasminogen-free human fibrinogen. A protein fraction was found with proteolytic activity which was heat labile and not attributable to plasmin. The activity was not potentiated by cysteine or inhibited by iodoacetamide. Studies of pH optima indicated a broad range of enzyme activity with peaks in both the acid and alkaline region. Cathepsin A activity was detected in the platelet protease fraction by hydrolysis of the synthetic substrate N-carbobenzoxy-alpha-L-glutamyl-L-tyrosine. Similar proteolytic activity was found when the proteins derived from isolated platelet granules were examined. The results indicate that human platelets possess potent intracellular proteolytic enzymes. The relationship of this proteolytic activity to the hemostatic process is discussed.

摘要

从人血小板中提取的可溶性蛋白质通过二乙氨基乙基纤维素柱色谱进行分级分离，并使用三种底物对蛋白酶活性进行分析：变性牛血红蛋白、α-酪蛋白和纯化的无纤溶酶原人纤维蛋白原。发现一种具有蛋白水解活性的蛋白质组分，其对热不稳定且不归因于纤溶酶。该活性不受半胱氨酸增强，也不受碘乙酰胺抑制。最适pH研究表明酶活性范围广泛，在酸性和碱性区域均有峰值。通过合成底物N-苄氧羰基-α-L-谷氨酰-L-酪氨酸的水解，在血小板蛋白酶组分中检测到组织蛋白酶A活性。当检查从分离的血小板颗粒中获得的蛋白质时，发现了类似的蛋白水解活性。结果表明人血小板拥有强大的细胞内蛋白酶。讨论了这种蛋白水解活性与止血过程的关系。

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J Clin Invest. 1967 Aug;46(8):1380-9. doi: 10.1172/JCI105630.
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On the proteolytic enzymes of animal tissues. X. Beef spleen cathepsin C.论动物组织中的蛋白水解酶。十、牛脾组织蛋白酶C。
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The purification and properties of a proteolytic enzyme, rabbit cathepsin E, and further studies on rabbit cathepsin D.一种蛋白水解酶——兔组织蛋白酶E的纯化及性质，以及对兔组织蛋白酶D的进一步研究。
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Enzyme system in rat leucocyte granules which degrades insoluble collagen.大鼠白细胞颗粒中降解不溶性胶原蛋白的酶系统。
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Platelet-dependent generation of chemotactic activity in serum.血清中血小板依赖性趋化活性的产生
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7
Increased vascular permeability produced by human platelet granule cationic extract.人血小板颗粒阳离子提取物产生的血管通透性增加
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The actin and myosin filaments of human and bovine blood platelets.人类和牛血血小板的肌动蛋白丝和肌球蛋白丝。
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[Current findings in the pathogenesis of the shock process in traumatology].[创伤学中休克过程发病机制的当前研究结果]
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