Nachman R L, Weksler B, Ferris B
J Clin Invest. 1972 Mar;51(3):549-56. doi: 10.1172/JCI106843.
Human platelet acid extract obtained from both whole platelets and from isolated subcellular granules was partially purified by DEAE-cellulose chromatography and Sephadex gel filtration. The heat-stable, nondialyzable cationic protein fraction with a mol wt of approximately 30,000 produced a biphasic increase in vascular permeability in rabbit skin and also had antiheparin activity. The acute (15 min) increase in vascular permeability was blocked by prior treatment of the animal with antihistamine and was characterized histologically by edema of perivascular tissues and dilation of capillaries and veinules. The delayed (3 hr) permeability effect was not blocked by antihistamine and was characterized histologically by leukocytic infiltration into the skin. The experiments described suggest that human platelet lysosomal release of cationic proteins may increase vascular permeability by several mechanisms including endogenous histamine release as well as delayed chemotaxis.
从全血小板和分离出的亚细胞颗粒中获得的人血小板酸提取物，通过DEAE - 纤维素色谱法和葡聚糖凝胶过滤法进行了部分纯化。分子量约为30,000的热稳定、不可透析的阳离子蛋白组分，在兔皮中引起了血管通透性的双相增加，并且还具有抗肝素活性。血管通透性的急性（15分钟）增加可通过预先用抗组胺药处理动物来阻断，组织学特征为血管周围组织水肿以及毛细血管和小静脉扩张。延迟（3小时）的通透性效应不能被抗组胺药阻断，组织学特征为白细胞浸润到皮肤中。所描述的实验表明，人血小板溶酶体释放阳离子蛋白可能通过多种机制增加血管通透性，包括内源性组胺释放以及延迟趋化作用。
