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[在十二烷基硫酸钠存在下利用聚丙烯酰胺凝胶电泳分离大鼠腺垂体激素]

[Separation of the hormones of the adenohypophysis of rats by use of electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulfate].

作者信息

Martynenko F P, Sakhno T O

出版信息

Ukr Biokhim Zh. 1975 Jan-Feb;47(1):122-5.

PMID:829
Abstract

A comparative study of rat adenohypophysis extract and its alcohol fractions was performed by two variants of the method of electrophoresis in polyacrylamide gel: at pH 9,5 and with the presence of sodium dodecyl sulphate at pH 7.2. With the presence of sodium dodecylsulphate four protein zones are found which in the order from the anode towards the cathode are identified as hemoglobin, somatotropin, lactotropin and albumin. It is shown that the somatotropin zone after the extract separation at pH 9.5 is inhomogeneous and consists of somatotropin and hemoglobin.

摘要

采用聚丙烯酰胺凝胶电泳法的两种变体,对大鼠垂体前叶提取物及其乙醇馏分进行了比较研究:一种是在pH 9.5条件下,另一种是在pH 7.2且存在十二烷基硫酸钠的条件下。在存在十二烷基硫酸钠的情况下,发现了四个蛋白质区带,从阳极到阴极依次鉴定为血红蛋白、生长激素、催乳素和白蛋白。结果表明,在pH 9.5条件下分离提取物后,生长激素区带是不均匀的,由生长激素和血红蛋白组成。

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