金黄色葡萄球菌经溶葡萄球菌酶处理后的细胞转染
Transfection of lysostaphin-treated cells of Staphylococcus aureus.
作者信息
Riggs H G, Rosenblum E D
出版信息
J Virol. 1969 Jan;3(1):33-7. doi: 10.1128/JVI.3.1.33-37.1969.
Abstract
After treatment with 1 unit of lysostaphin per ml for 3 min, two strains of Staphylococcus aureus, 233 and PS 44A HJD, were transfected with phenol-extracted deoxyribonucleic acid (DNA) from the staphylococcal bacteriophages, 53 and 44A HJD, respectively. The number of transfected cells was low in both systems, approximately two in 10(7) enzyme-treated cells. There was a saturation effect at high concentrations of DNA; optimal results were obtained at concentrations between 10 to 25 mug/ml. Growth curves and fluctuation tests indicated that cells of strain 44A HJD infected with phage, then converted to protoplasts by a 10-min treatment with lysostaphin, produce only one phage particle and lose their ability to lyse spontaneously in hypertonic media.
摘要
用每毫升1单位的溶葡萄球菌酶处理3分钟后，分别用来自葡萄球菌噬菌体53和44A HJD的酚提取脱氧核糖核酸（DNA）转染两株金黄色葡萄球菌，菌株233和PS 44A HJD。在这两个系统中转染细胞的数量都很低，在10⁷个酶处理细胞中约有两个。在高浓度DNA时存在饱和效应；在10至25微克/毫升的浓度下可获得最佳结果。生长曲线和波动试验表明，用噬菌体感染然后用溶葡萄球菌酶处理10分钟转化为原生质体的44A HJD菌株细胞仅产生一个噬菌体颗粒，并失去在高渗培养基中自发裂解的能力。
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