用从噬菌体R phi 6P分离的脱氧核糖核酸对球形红假单胞菌进行转化。
Transformation of Rhodopseudomonas sphaeroides with deoxyribonucleic acid isolated from bacteriophage R phi 6P.
作者信息
Tucker W T, Pemberton J M
出版信息
J Bacteriol. 1980 Jul;143(1):43-9. doi: 10.1128/jb.143.1.43-49.1980.
Abstract
The transformation of the photosynthetic bacterium Rhodopseudomonas sphaeroides with the circular genome of the penicillinase-encoding, temperate bacteriophage R phi 6P was demonstrated. The transformation was dependent on the infection of the recipient by another, apparently closely related, temperature bacteriophage, R phi 9. Optimum transformation occurred in the recipient cells already lysogenic for R phi 9 when superinfected with that bacteriophage at multiplicities of infection between 1 and 10 R phi 9 particles per recipient cell.
摘要
已证实，青霉素酶编码的温和噬菌体R phi 6P的环状基因组可转化光合细菌球形红假单胞菌。这种转化依赖于另一种明显密切相关的温和噬菌体R phi 9对受体的感染。当以每个受体细胞1至10个R phi 9颗粒的感染复数用该噬菌体进行超感染时，在已经对R phi 9呈溶原性的受体细胞中发生最佳转化。
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