用噬菌体脱氧核糖核酸转染金黄色葡萄球菌。
Transfection of Staphylococcus aureus with bacteriophage deoxyribonucleic acid.
作者信息
Sjöström J E, Lindberg M, Philipson L
出版信息
J Bacteriol. 1972 Jan;109(1):285-91. doi: 10.1128/jb.109.1.285-291.1972.
Abstract
Staphylococcus aureus cells of strain 8325 (N) are competent for phage deoxyribonucleic acid (DNA) when harvested in the early exponential growth phase. Phenotypic expression of the competence requires divalent cations, and calcium ions are most effective. Treatment of phage DNA with deoxyribonuclease completely destroys infectivity and heat-denaturated DNA is not infectious. The highest frequency of transfection is around 10(4) plaque-forming units per mug of DNA.
摘要
8325（N）菌株的金黄色葡萄球菌细胞在指数生长早期收获时具有摄取噬菌体脱氧核糖核酸（DNA）的能力。感受态的表型表达需要二价阳离子，其中钙离子最为有效。用脱氧核糖核酸酶处理噬菌体DNA会完全破坏其感染性，热变性的DNA也没有感染性。最高转染频率约为每微克DNA 10⁴个噬菌斑形成单位。
相似文献
1
Transfection of Staphylococcus aureus with bacteriophage deoxyribonucleic acid.用噬菌体脱氧核糖核酸转染金黄色葡萄球菌。
J Bacteriol. 1972 Jan;109(1):285-91. doi: 10.1128/jb.109.1.285-291.1972.
2
Competence for transfection in Staphylococcus aureus.金黄色葡萄球菌的转染能力。
J Bacteriol. 1973 Feb;113(2):576-85. doi: 10.1128/jb.113.2.576-585.1973.
3
Role of the phi 11 phage genome in competence of Staphylococcus aureus.φ11噬菌体基因组在金黄色葡萄球菌感受态中的作用。
J Bacteriol. 1974 Jul;119(1):19-32. doi: 10.1128/jb.119.1.19-32.1974.
4
Large-scale production of lambda bacteriophage and purified lambda deoxyribonucleic acid.λ噬菌体的大规模生产及纯化的λ脱氧核糖核酸
Appl Microbiol. 1972 Apr;23(4):791-5. doi: 10.1128/am.23.4.791-795.1972.
5
Transfection in Agrobacterium tumefaciens.农杆菌介导的转染
J Virol. 1972 Jul;10(1):17-22. doi: 10.1128/JVI.10.1.17-22.1972.
6
Source of the nonlinear dependence of bacteriophage SP82 transfection on deoxyribonucleic acid concentration.噬菌体SP82转染对脱氧核糖核酸浓度非线性依赖的来源。
J Virol. 1971 Jun;7(6):749-52. doi: 10.1128/JVI.7.6.749-752.1971.
7
Factors affecting competence for transformation in Staphylococcus aureus.影响金黄色葡萄球菌转化能力的因素。
J Bacteriol. 1974 Apr;118(1):155-64. doi: 10.1128/jb.118.1.155-164.1974.
8
Morphology and physical properties of Staphylococcus bacteriophage P11-M15.葡萄球菌噬菌体P11-M15的形态学与物理特性
J Virol. 1972 Apr;9(4):664-71. doi: 10.1128/JVI.9.4.664-671.1972.
9
Transformation of chromosomal and plasmid characters in Staphylococcus aureus.金黄色葡萄球菌中染色体和质粒特征的转化
J Bacteriol. 1972 Feb;109(2):844-7. doi: 10.1128/jb.109.2.844-847.1972.
10
Infectious DNA preparations from T2 and T4 bacteriophages.来自T2和T4噬菌体的传染性DNA制剂。
Virology. 1971 Nov;46(2):192-9. doi: 10.1016/0042-6822(71)90022-5.
引用本文的文献
1
Growth-Dependent Predation and Generalized Transduction of Antimicrobial Resistance by Bacteriophage.噬菌体对生长依赖性捕食作用和抗生素耐药性的广义转导。
mSystems. 2022 Apr 26;7(2):e0013522. doi: 10.1128/msystems.00135-22. Epub 2022 Mar 21.
2
Cross-Genus "Boot-Up" of Synthetic Bacteriophage in Staphylococcus aureus by Using a New and Efficient DNA Transformation Method.利用新型高效 DNA 转化方法实现金黄色葡萄球菌中合成噬菌体的跨属“启动”。
Appl Environ Microbiol. 2022 Feb 8;88(3):e0148621. doi: 10.1128/AEM.01486-21. Epub 2021 Nov 24.
3
Revisiting the Mechanisms Involved in Calcium Chloride Induced Bacterial Transformation.重新审视氯化钙诱导细菌转化所涉及的机制。
Front Microbiol. 2017 Nov 7;8:2169. doi: 10.3389/fmicb.2017.02169. eCollection 2017.
4
Recovery of competence in calcium-limited Azotobacter vinelandii.在钙限制条件下维涅兰德固氮菌能力的恢复。
J Bacteriol. 1981 Apr;146(1):33-40. doi: 10.1128/jb.146.1.33-40.1981.
5
Genetic transformation of Rhodopseudomonas sphaeroides by plasmid DNA.球形红假单胞菌的质粒DNA遗传转化
J Bacteriol. 1982 Oct;152(1):89-97. doi: 10.1128/jb.152.1.89-97.1982.
6
Genetic transformation in Staphylococcus aureus: isolation and characterization of a competence-conferring factor from bacteriophage 80 alpha lysates.金黄色葡萄球菌中的基因转化：从噬菌体80α裂解物中分离并鉴定一种赋予感受态的因子。
J Bacteriol. 1981 Oct;148(1):301-7. doi: 10.1128/jb.148.1.301-307.1981.
7
Genetic transformation in Staphylococcus aureus: demonstration of a competence-conferring factor of bacteriophage origin in bacteriophage 80 alpha lysates.金黄色葡萄球菌中的遗传转化：噬菌体80α裂解物中噬菌体来源的能力赋予因子的证明。
J Bacteriol. 1981 Oct;148(1):294-300. doi: 10.1128/jb.148.1.294-300.1981.
8
Genetic mapping in phage group 2 Staphylococcus aureus.噬菌体群2型金黄色葡萄球菌的基因图谱分析
Infect Immun. 1980 Feb;27(2):532-41. doi: 10.1128/iai.27.2.532-541.1980.
9
Transformation of chromosomal and plasmid characters in Staphylococcus aureus.金黄色葡萄球菌中染色体和质粒特征的转化
J Bacteriol. 1972 Feb;109(2):844-7. doi: 10.1128/jb.109.2.844-847.1972.
10
Transfection mediated by Mycoplasmatales viral DNA.支原体目病毒DNA介导的转染
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3423-7. doi: 10.1073/pnas.69.11.3423.
本文引用的文献
1
THE INFECTION OF SUB-CELLULAR ESCHERICHIA COLI, STRAIN B, WITH A DNA PREPARATION FROM T2 BACTERIOPHAGE.用T2噬菌体的DNA制剂感染亚细胞大肠杆菌B菌株。
Proc Natl Acad Sci U S A. 1957 Nov 15;43(11):939-47. doi: 10.1073/pnas.43.11.939.
2
INFECTION OF PROTOPLASTS BY DISRUPTED T2 VIRUS.被裂解的T2病毒对原生质体的感染
Proc Natl Acad Sci U S A. 1957 Aug 15;43(8):694-701. doi: 10.1073/pnas.43.8.694.
3
BACTERIOPHAGE DEOXYRIBONUCLEATE INFECTION OF COMPETENT BACILLUS SUBTILIS.感受态枯草芽孢杆菌的噬菌体脱氧核糖核酸感染
J Bacteriol. 1965 Mar;89(3):782-90. doi: 10.1128/jb.89.3.782-790.1965.
4
INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82.从枯草芽孢杆菌噬菌体SP82中分离出的DNA的感染性
J Mol Biol. 1964 Dec;10:438-51. doi: 10.1016/s0022-2836(64)80065-6.
5
BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA.腺病毒增殖的生化研究，VI. 高度纯化的致瘤人类腺病毒及其DNA的特性
Proc Natl Acad Sci U S A. 1964 Jun;51(6):1251-9. doi: 10.1073/pnas.51.6.1251.
6
NUTRITIONAL MUTATIONS AND TRANSDUCTION BY ULTRAVIOLET-INACTIVATED PHAGE IN STAPHYLOCOCCUS AUREUS.
J Bacteriol. 1964 Jul;88(1):226-32. doi: 10.1128/jb.88.1.226-232.1964.
7
PROPERTIES OF INFECTIOUS DEOXYRIBONUCLEIC ACID FROM T1 AND LAMBDA BACTERIOPHAGE.来自T1和λ噬菌体的传染性脱氧核糖核酸的特性
J Biol Chem. 1964 Jan;239:285-9.
8
ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS.通过转导分析影响金黄色葡萄球菌青霉素酶形成的突变
J Gen Microbiol. 1963 Oct;33:121-36. doi: 10.1099/00221287-33-1-121.
9
INCORPORATION OF DEOXYRIBONUCLEIC ACID IN THE BACILLUS SUBTILIS TRANSFORMATION SYSTEM.脱氧核糖核酸在枯草芽孢杆菌转化系统中的掺入
J Bacteriol. 1963 Sep;86(3):392-400. doi: 10.1128/jb.86.3.392-400.1963.
10
The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg.用从噬菌体λ-dg分离的脱氧核糖核酸对大肠杆菌进行转化。
J Mol Biol. 1960 Dec;2:392-415. doi: 10.1016/s0022-2836(60)80050-2.
Zotero 插件