用噬菌体脱氧核糖核酸转染金黄色葡萄球菌。
Transfection of Staphylococcus aureus with bacteriophage deoxyribonucleic acid.
作者信息
Sjöström J E, Lindberg M, Philipson L
出版信息
J Bacteriol. 1972 Jan;109(1):285-91. doi: 10.1128/jb.109.1.285-291.1972.
Abstract
Staphylococcus aureus cells of strain 8325 (N) are competent for phage deoxyribonucleic acid (DNA) when harvested in the early exponential growth phase. Phenotypic expression of the competence requires divalent cations, and calcium ions are most effective. Treatment of phage DNA with deoxyribonuclease completely destroys infectivity and heat-denaturated DNA is not infectious. The highest frequency of transfection is around 10(4) plaque-forming units per mug of DNA.
摘要
8325（N）菌株的金黄色葡萄球菌细胞在指数生长早期收获时具有摄取噬菌体脱氧核糖核酸（DNA）的能力。感受态的表型表达需要二价阳离子，其中钙离子最为有效。用脱氧核糖核酸酶处理噬菌体DNA会完全破坏其感染性，热变性的DNA也没有感染性。最高转染频率约为每微克DNA 10⁴个噬菌斑形成单位。
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