Catterall J F, Stein J P, Lai E C, Woo S L, Dugaiczyk A, Mace M L, Means A R, O'Malley B W
Nature. 1979 Mar 22;278(5702):323-7. doi: 10.1038/278323a0.
A 15-kilobase pair EcoRI chick DNA fragment, containing both the termination codon UGA and the 5'-portion of the structural ovomucoid gene, has been cloned in lambda phage Charon 4A by in vitro packaging. Restriction mapping and electron microscopic analyses of this cloned DNA have revealed that the structural ovomucoid gene sequences are separated by at least six intervening sequences.
一个包含终止密码子UGA和卵类粘蛋白结构基因5'端部分的15千碱基对的EcoRI鸡DNA片段,已通过体外包装克隆到λ噬菌体Charon 4A中。对该克隆DNA的限制性图谱分析和电子显微镜分析表明,卵类粘蛋白结构基因序列被至少六个间隔序列隔开。