Tervo Timo, Joó Ferenc, Huikuri Kauko T, Toth Ida, Palkama Arto
Department of Anatomy, University of Helsinki, Helsinki Finland Department of Surgery, Meilahti Hospital Medical School, Helsinki, Finland Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center, Szeged Hungary.
Pain. 1979 Feb;6(1):57-70. doi: 10.1016/0304-3959(79)90140-4.
Fine structure of nerve fibres and terminals in the rat cornea was studied after fixation with glutaraldehyde and osmium tetroxide or with potassium permanganate. Superior cervical and/or ciliary ganglionectomy as well as ophthalmic neurotomy were performed in order to verify the origin of the corneal nerves. In the control corneas axon profiles with granular vesicles were observed in the stroma. Other axons containing agranular vesicles were found both in the stroma and in the epithelium. Superior cervical ganglionectomy abolished only the axons containing granular vesicles but did not affect the corneal blink reflex. Ciliary ganglionectomy induced more conspicuous changes in both stromal and intraepithelial axon profiles. The corneal reflex was, however, normal. Some of the axons containing agranular vesicles also showed degenerative changes. However, intact axon profiles with agranular vesicles were seen even after extirpation of both the ciliary ganglion and the superior cervical ganglion (combined autonomic ganglionectomy). On the other hand, all axon profiles with agranular vesicles disappeared after coagulation of the ophthalmic and maxillary nerves (sensory denervation). This operation destroyed most of the axons in all parts of the cornea and abolished the blink reflex. It is concluded that the axon profiles in the corneal epithelium, containing agranular vesicles, are sensory axons of the trigeminal nerve. A possible role of the vesicles in sensory nerves is discussed.
用戊二醛和四氧化锇或高锰酸钾固定后，研究大鼠角膜神经纤维和终末的精细结构。为了验证角膜神经的起源，进行了颈上神经节和/或睫状神经节切除术以及眼神经切断术。在对照角膜中，在基质中观察到有颗粒小泡的轴突轮廓。在基质和上皮中均发现了其他含有无颗粒小泡的轴突。颈上神经节切除术仅消除了含有颗粒小泡的轴突，但不影响角膜眨眼反射。睫状神经节切除术在基质和上皮内轴突轮廓上引起更明显的变化。然而，角膜反射正常。一些含有无颗粒小泡的轴突也显示出退行性变化。然而，即使在切除睫状神经节和颈上神经节（联合自主神经节切除术）后，仍可见到带有无颗粒小泡的完整轴突轮廓。另一方面，眼神经和上颌神经凝固（感觉去神经支配）后，所有含有无颗粒小泡的轴突轮廓均消失。该手术破坏了角膜所有部位的大部分轴突，并消除了眨眼反射。结论是，角膜上皮中含有无颗粒小泡的轴突轮廓是三叉神经的感觉轴突。讨论了小泡在感觉神经中的可能作用。
