磷酸烯醇丙酮酸对豌豆种子磷酸果糖激酶的调节作用。
The regulation of pea-seed phosphofructokinase by phosphoenolpyruvate.
作者信息
Kelly G J, Turner J F
出版信息
Biochem J. 1969 Nov;115(3):481-7. doi: 10.1042/bj1150481.
Abstract
- Pea-seed phosphofructokinase was purified 27-fold by a combination of fractionation with ethanol and ammonium sulphate. Under the conditions of assay, the enzyme was strongly inhibited by phosphoenolpyruvate. This inhibition was reversed by increasing the concentration of fructose 6-phosphate or magnesium chloride, or by lowering the ATP concentration. 2. Citrate, ADP and AMP inhibited phosphofructokinase and increased the sensitivity to phosphoenolpyruvate inhibition. Sulphate and inorganic phosphate stimulated the enzyme activity and decreased the sensitivity to phosphoenolpyruvate. 3. In the presence of inorganic phosphate and low concentrations of ATP, inhibition by phosphoenolpyruvate ceased and phosphoenolpyruvate became stimulatory. 4. The possible significance of these results in the control of plant carbohydrate metabolism is discussed.
摘要
- 通过乙醇分级分离和硫酸铵沉淀相结合的方法，豌豆种子磷酸果糖激酶被纯化了27倍。在测定条件下，该酶受到磷酸烯醇丙酮酸的强烈抑制。增加6-磷酸果糖或氯化镁的浓度，或降低ATP浓度可逆转这种抑制作用。2. 柠檬酸、ADP和AMP抑制磷酸果糖激酶，并增加对磷酸烯醇丙酮酸抑制的敏感性。硫酸盐和无机磷酸盐刺激酶活性，并降低对磷酸烯醇丙酮酸的敏感性。3. 在存在无机磷酸盐和低浓度ATP的情况下，磷酸烯醇丙酮酸的抑制作用停止，并且磷酸烯醇丙酮酸变得具有刺激作用。4. 讨论了这些结果在植物碳水化合物代谢控制中的可能意义。
相似文献
1
The regulation of pea-seed phosphofructokinase by phosphoenolpyruvate.磷酸烯醇丙酮酸对豌豆种子磷酸果糖激酶的调节作用。
Biochem J. 1969 Nov;115(3):481-7. doi: 10.1042/bj1150481.
2
Inhibition of pea-seed phosphofructokinase by phosphoenolpyruvate.磷酸烯醇丙酮酸对豌豆种子磷酸果糖激酶的抑制作用。
Biochem Biophys Res Commun. 1968 Jan 25;30(2):195-9. doi: 10.1016/0006-291x(68)90470-1.
3
Purification and properties of sheep liver phosphofructokinase.绵羊肝脏磷酸果糖激酶的纯化及性质
Biochem J. 1969 Jun;113(2):235-42. doi: 10.1042/bj1130235.
4
Citrate and the regulation of adipose-tissue phosphofructokinase.柠檬酸盐与脂肪组织磷酸果糖激酶的调节
Biochem J. 1966 Aug;100(2):420-3. doi: 10.1042/bj1000420.
5
The regulation of pea-seed phosphofructokinase by 6-phosphogluconate, 3-phosphoglycerate, 2-phosphoglycerate and phosphoenolpyruvate.6-磷酸葡萄糖酸、3-磷酸甘油酸、2-磷酸甘油酸和磷酸烯醇丙酮酸对豌豆种子磷酸果糖激酶的调节作用
Biochim Biophys Acta. 1970 Jun;208(3):360-7. doi: 10.1016/0304-4165(70)90207-2.
6
Properties of rat lens phosphofructokinase.大鼠晶状体磷酸果糖激酶的特性
Invest Ophthalmol. 1976 Apr;15(4):279-87.
7
PROPERTIES OF PHOSPHOFRUCTOKINASE FROM RAT LIVER AND THEIR RELATION TO THE CONTROL OF GLYCOLYSIS AND GLUCONEOGENESIS.大鼠肝脏磷酸果糖激酶的特性及其与糖酵解和糖异生调控的关系
Biochem J. 1965 Jun;95(3):868-75. doi: 10.1042/bj0950868.
8
Some properties of phosphofructokinase from kidney cortex and their relation to glucose metabolism.肾皮质磷酸果糖激酶的一些特性及其与葡萄糖代谢的关系。
Biochem J. 1967 Jul;104(1):296-9. doi: 10.1042/bj1040296.
9
Factors affecting the inhibition of phosphofructokinase activity of Schistosoma mansoni by trivalent organic antimonials.三价有机锑化合物对曼氏血吸虫磷酸果糖激酶活性抑制作用的影响因素
Biochem Pharmacol. 1966 Aug;15(8):1197-211. doi: 10.1016/0006-2952(66)90285-1.
10
A comparison of the properties of the phosphofructokinases of the fat body and flight muscle of the adult male desert locust.成年雄性沙漠蝗虫脂肪体和飞行肌中磷酸果糖激酶特性的比较。
Biochem J. 1969 Feb;111(3):365-9. doi: 10.1042/bj1110365.
引用本文的文献
1
Control of glycolysis by orthophosphate and adenosine triphosphate in soluble extracts of germinating pea seeds.发芽豌豆种子可溶性提取物中 orthophosphate 和三磷酸腺苷对糖酵解的控制。
Planta. 1972 Mar;108(1):29-38. doi: 10.1007/BF00386504.
2
The control of glycolysis in aged slices of carrot root tissue.老龄胡萝卜根组织切片中糖酵解的控制。
Planta. 1974 Sep;118(3):211-24. doi: 10.1007/BF00384777.
3
Pathways of carbohydrate fermentation in the roots of marsh plants.沼泽植物根中碳水化合物发酵途径。
Planta. 1979 Jan;146(3):327-34. doi: 10.1007/BF00387805.
4
Role of ATP in nitrite reduction in roots of wheat and pea.三磷酸腺苷在小麦和豌豆根中亚硝酸盐还原中的作用。
Planta. 1981 Jul;152(3):234-8. doi: 10.1007/BF00385149.
5
Cytosolic Phosphofructokinase from Spinach Leaves : II. Affinity for Mg and Nucleoside Phosphates.菠菜叶细胞质磷酸果糖激酶：II. 对 Mg 和核苷磷酸的亲和力。
Plant Physiol. 1989 Aug;90(4):1506-12. doi: 10.1104/pp.90.4.1506.
6
Cytosolic phosphofructokinase from spinach leaves : I. Purification, characteristics, and regulation.菠菜叶细胞质磷酸果糖激酶：I. 纯化、特性和调控。
Plant Physiol. 1989 Aug;90(4):1498-505. doi: 10.1104/pp.90.4.1498.
7
Metabolic Changes in Gladiolus Cormels During the Break of Dormancy: The Role of Dark CO(2) Fixation.唐菖蒲子球休眠解除过程中的代谢变化：暗固定二氧化碳的作用
Plant Physiol. 1981 Nov;68(5):1105-9. doi: 10.1104/pp.68.5.1105.
8
Effect of salts on the activity of carrot phosphofructokinase.盐对胡萝卜磷酸果糖激酶活性的影响。
Plant Physiol. 1980 Nov;66(5):973-7. doi: 10.1104/pp.66.5.973.
9
Chloroplast Phosphofructokinase: II. Partial Purification, Kinetic and Regulatory Properties.叶绿体磷酸果糖激酶：二、部分纯化、动力学和调节性质。
Plant Physiol. 1977 Aug;60(2):295-9. doi: 10.1104/pp.60.2.295.
10
Chloroplast phosphofructokinase: I. Proof of phosphofructokinase activity in chloroplasts.叶绿体磷酸果糖激酶：I. 叶绿体中磷酸果糖激酶活性的证明。
Plant Physiol. 1977 Aug;60(2):290-4. doi: 10.1104/pp.60.2.290.
本文引用的文献
1
Short-term Changes in Hexose Phosphates and ATP in Intact Cells of Acer pseudoplatanus L. Subjected to Anoxia.缺氧条件下美洲槭完整细胞中己糖磷酸和 ATP 的短期变化。
Plant Physiol. 1968 Jun;43(6):948-52. doi: 10.1104/pp.43.6.948.
2
Phosphofructokinase and the Pasteur effect.磷酸果糖激酶与巴斯德效应。
Biochem Biophys Res Commun. 1962 Feb 20;7:10-5. doi: 10.1016/0006-291x(62)90134-1.
3
A COMPARISON OF THE KINETIC PROPERTIES OF PHOSPHOFRUCTOKINASE FROM BACTERIAL, PLANT AND ANIMAL SOURCES.来自细菌、植物和动物源的磷酸果糖激酶动力学特性比较
Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1964 May 11;248:185-94. doi: 10.1007/BF00246673.
4
THE STABILITY CONSTANTS OF METAL-ADENINE NUCLEOTIDE COMPLEXES.金属 - 腺嘌呤核苷酸络合物的稳定常数
Biochemistry. 1964 Jan;3:18-26. doi: 10.1021/bi00889a005.
5
End-product inhibition of yeast phosphofructokinase by ATP.ATP对酵母磷酸果糖激酶的终产物抑制作用。
Biochem Biophys Res Commun. 1963 Jul 18;12:140-5. doi: 10.1016/0006-291x(63)90250-x.
6
Allosteric proteins and cellular control systems.别构蛋白与细胞控制系统。
J Mol Biol. 1963 Apr;6:306-29. doi: 10.1016/s0022-2836(63)80091-1.
7
The biosynthesis of sucrose.蔗糖的生物合成。
Biochem J. 1957 Nov;67(3):450-6. doi: 10.1042/bj0670450.
8
Studies on the enzyme enolase. I. Equilibrium studies.烯醇化酶的研究。I. 平衡研究。
J Biol Chem. 1957 Jul;227(1):301-12.
9
Phosphonexokinase in higher plants.高等植物中的磷酸己糖激酶
J Biol Chem. 1952 May;197(1):89-96.
10
Interaction between energy charge and metabolite modulation in the regulation of enzymes of amphibolic sequences. Phosphofructokinase and pyruvate dehydrogenase.兼性代谢途径中酶调控过程中能量电荷与代谢物调节之间的相互作用。磷酸果糖激酶和丙酮酸脱氢酶。
Biochemistry. 1968 Nov;7(11):4041-5. doi: 10.1021/bi00851a035.
Zotero 插件