β-珠蛋白mRNA的3'非编码区对于体外翻译并非必不可少。
The 3' noncoding region of beta-globin mRNA is not essential for in vitro translation.
作者信息
Kronenberg M N, Roberts B E, Efstratiadis A
出版信息
Nucleic Acids Res. 1979 Jan;6(1):153-66. doi: 10.1093/nar/6.1.153.
Abstract
Rabbit beta globin DNA sequence, excised from plasmid pbetaG1, directs in vitro synthesis of beta-globin in a transcription-translation cell-free system, even after specific elimination of the entire 3'-noncoding region. A DNA restriction fragment carrying this 3' noncoding region and hybridized to globin mRNA cannot arrest the cell-free translation of beta-globin mRNA.
摘要
从质粒pbetaG1中切下的兔β-珠蛋白DNA序列，即使在特异性去除整个3'-非编码区后，仍能在无细胞转录-翻译系统中指导β-珠蛋白的体外合成。携带该3'非编码区并与珠蛋白mRNA杂交的DNA限制性片段不能阻止β-珠蛋白mRNA的无细胞翻译。
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