Kronenberg M N, Roberts B E, Efstratiadis A
Nucleic Acids Res. 1979 Jan;6(1):153-66. doi: 10.1093/nar/6.1.153.
Rabbit beta globin DNA sequence, excised from plasmid pbetaG1, directs in vitro synthesis of beta-globin in a transcription-translation cell-free system, even after specific elimination of the entire 3'-noncoding region. A DNA restriction fragment carrying this 3' noncoding region and hybridized to globin mRNA cannot arrest the cell-free translation of beta-globin mRNA.