抗生素偏端霉素A的吡咯甲酰胺基团对其与DNA中AT碱基对结合特异性贡献的定量评估。
Quantitative estimation of the contribution of pyrrolcarboxamide groups of the antibiotic distamycin A into specificity of its binding to DNA AT pairs.
作者信息
Krylov A S, Grokhovsky S L, Zasedatelev A S, Zhuze A L, Gursky G V, Gottikh B P
出版信息
Nucleic Acids Res. 1979 Jan;6(1):289-304. doi: 10.1093/nar/6.1.289.
Abstract
Interaction of DNA with the analogs of the antibiotic distamycin A having different numbers of pyrrolcarboxamide groups and labeled with dansyl was studied. The binding isoterms of the analogs to synthetic polydeoxyribonucleotides were obtained. Analysis of the experimental data leads to the following conclusions: (1) the free energy of binding of the analogs to poly(dA).poly(dT) depends linearly on the number of amide groups in the molecule of the analog whereas attachment of each pyrrolcarboxamide group produces changes of 2 kcal/mole in the free energy; (2) attachment of a pyrrolcarboxamide unit to the GC pair results in the free energy change of 0.95 kcal/mole; (3) the binding of analogs to poly(dA).poly(dT) is a cooperative process, presumbly, dependent on conformational changes induced by the binding of analogs to DNA.
摘要
研究了DNA与具有不同数量吡咯甲酰胺基团且用丹磺酰标记的抗生素偏端霉素A类似物之间的相互作用。获得了这些类似物与合成多脱氧核糖核苷酸的结合等温线。对实验数据的分析得出以下结论：(1) 类似物与聚（dA）·聚（dT）的结合自由能线性依赖于类似物分子中酰胺基团的数量，而每个吡咯甲酰胺基团的附着会使自由能产生2千卡/摩尔的变化；(2) 一个吡咯甲酰胺单元附着到GC对会导致自由能变化0.95千卡/摩尔；(3) 类似物与聚（dA）·聚（dT）的结合是一个协同过程，推测这取决于类似物与DNA结合诱导的构象变化。
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Quantitative estimation of the contribution of pyrrolcarboxamide groups of the antibiotic distamycin A into specificity of its binding to DNA AT pairs.抗生素偏端霉素A的吡咯甲酰胺基团对其与DNA中AT碱基对结合特异性贡献的定量评估。
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Structures for the polynucleotide complexes poly(dA) with poly (dT) and poly(dT) with poly(dA) with poly (dT).聚（dA）与聚（dT）以及聚（dT）与聚（dA）和聚（dT）形成的多核苷酸复合物的结构
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