α-鹅膏蕈碱抗性仓鼠细胞中聚合酶II对低分子量RNA组分A、C和D的合成
Synthesis of low molecular weight RNA components A, C and D by polymerase II in alpha-amanitin-resistant hamster cells.
作者信息
Jensen E G, Hellung-Larsen P, Frederiksen S
出版信息
Nucleic Acids Res. 1979 Jan;6(1):321-30. doi: 10.1093/nar/6.1.321.
Abstract
In an attempt to establish which RNA polymerase catalyzes the synthesis of the low molecular weight RNA components A, C and D, Ama 1 cells (mutant Chinese hamster cells) were used in experiments with addition of alpha-amanitin. Ama 1 cells contain an altered RNA polymerase II which is 800 times more resistant towards inhibition by alpha-amanitin than the wild type enzyme. Alpha-amanitin (up to 200 microgram/ml) added to these cells does not affect the synthesis of the low molecular weight RNAs A, C and D. These data together with our previous data showing that alpha-amanitin (0.5 - 5.0 microgram/ml) preferentially inhibits the synthesis of A, C and D in normal cells indicate that RNA polymerase II catalyzes the synthesis of the low molecular weight RNA components A, C and D.
摘要
为了确定哪种RNA聚合酶催化低分子量RNA组分A、C和D的合成，在添加α-鹅膏蕈碱的实验中使用了Ama 1细胞（中国仓鼠突变细胞）。Ama 1细胞含有一种改变的RNA聚合酶II，它对α-鹅膏蕈碱抑制作用的抗性比野生型酶高800倍。添加到这些细胞中的α-鹅膏蕈碱（高达200微克/毫升）不会影响低分子量RNA A、C和D的合成。这些数据以及我们之前的数据表明，α-鹅膏蕈碱（0.5 - 5.0微克/毫升）优先抑制正常细胞中A、C和D的合成，这表明RNA聚合酶II催化低分子量RNA组分A、C和D的合成。
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