Bardoń A, Slerakowska H, Shugar D
Clin Chim Acta. 1976 Mar 15;67(3):231-43. doi: 10.1016/0009-8981(76)90331-4.
Acid-thermostable ribonucleases were isolated from human pancreas, duodenal contents, liver, spleen, serum and urine, and purified 15--1000-fold. The pH optima, ionic requirements, and some of the specificity requirements, of these enzymes were investigated. The isolated enzymes formed two distinct groups: (a) The ribonucleases of the pancreas, duodenal contents and fraction A of serum and urine exhibit a pH optimum of 8.5, are inhibited by An2+ and Cu2+, and relatively rapidly hydrolyze the synthetic substrate uridine 3'-(alpha-naphthylphosphate); (b) the ribonucleases of the liver and spleen, and of fractions B of the serum and urine, with a pH optimum of 7, are less sensitive to An2+ and Cu2+, and exhibit negligible activity versus uridine 3'-(alpha-naphthylphosphate). Determination of the serum level of pancreatic-type ribonuclease activity, with the use of uridine 3'-(alpha-naphthylphosphate) or RNA as substrates, appears to be a valid diagnostic tool for pancreatic fibrosis in children.
从人胰腺、十二指肠内容物、肝脏、脾脏、血清和尿液中分离出酸稳定核糖核酸酶，并将其纯化了15至1000倍。对这些酶的最适pH值、离子需求以及一些特异性需求进行了研究。分离出的酶形成了两个不同的组：(a)胰腺、十二指肠内容物以及血清和尿液的A组分中的核糖核酸酶，其最适pH值为8.5，受An2+和Cu2+抑制，并且相对快速地水解合成底物尿苷3'-(α-萘基磷酸酯)；(b)肝脏和脾脏以及血清和尿液的B组分中的核糖核酸酶，其最适pH值为7，对An2+和Cu2+不太敏感，并且对尿苷3'-(α-萘基磷酸酯)的活性可忽略不计。以尿苷3'-(α-萘基磷酸酯)或RNA为底物测定血清中胰腺型核糖核酸酶活性水平，似乎是诊断儿童胰腺纤维化的有效工具。
