Bardoń A, Shugar D
Clin Chim Acta. 1980 Feb 14;101(1):17-24. doi: 10.1016/0009-8981(80)90051-0.
Alkaline acid- and thermo-stable ribonuclease was isolated from human saliva of healthy subjects, children with cystic fibrosis, and from parents of the latter. The enzyme was purified about 180-200 times, and its ionic requirements, and specificity towards various substrates, characterized and compared with those for human pancreatic ribonuclease. The level of acid- and thermo-stable ribonuclease activity was five times higher in terms of enzyme level per ml saliva, and three times higher in terms of salivary protein, for 62% of children with cystic fibrosis, and for 73% of carrier subjects, relative to co-nrol groups. Assay of the level of this ribonuclease activity appears to be a promising diagnostic tool for detection of heterozygote carriers.
从健康受试者、患有囊性纤维化的儿童及其父母的唾液中分离出碱性、耐酸且耐热的核糖核酸酶。该酶被纯化了约180 - 200倍，对其离子需求以及对各种底物的特异性进行了表征，并与人类胰腺核糖核酸酶进行了比较。相对于对照组，62%的囊性纤维化儿童和73%的携带者受试者，其每毫升唾液中的酸热稳定核糖核酸酶活性水平在酶水平方面高出五倍，在唾液蛋白方面高出三倍。检测这种核糖核酸酶活性水平似乎是一种有前景的检测杂合子携带者的诊断工具。
