Bardoń A, Sierakowska H, Shugar D
Biochim Biophys Acta. 1976 Jul 8;438(2):461-73. doi: 10.1016/0005-2744(76)90262-x.
Purified acid-thermostable ribonuclease (Ribonucleate 3'-pyrimidino-oligonucleotidohydrolase, EC 3.1.4.22) from human pancreas degrades double-stranded RNA at 2% the rate for single-stranded RNA. The activities against single-stranded RNA and double-stranded RNA were shown to be due to a single enzyme with properties similar to bovine pancreatic RNAase A. For purposes of comparison the activities against double-stranded RNA of crystalline ribonucleases of the whale, rat and cow were assayed and found to be 0.4%, 0.03% and 0.003%, respectively, of their activities against single-stranded RNA. Both human serum and urine contain RNAse components of pancreatic origin which hydrolyze double-stranded RNA at 2% and 0.4%, respectively, of the rates against single-stranded RNA. By contrast, purified acid-thermostable RNAases from human spleen and liver hydrlyze double-stranded RNA at least 20-fold more slowly than human pancreatic RNAase, relative to the corresponding rates against single-stranded RNA. The human pancreatic and serum enzymes exhibit appreciable activity against the poly(C) component of the double-stranded poly(I)-poly(C); they also attack poly(C) itself at approximately 25 times the rate for poly(U) and at more than 50 times the rate for single-stranded RNA.
从人胰腺中纯化得到的酸稳定核糖核酸酶（核糖核酸3'-嘧啶基寡核苷酸水解酶，EC 3.1.4.22）降解双链RNA的速度是单链RNA的2%。针对单链RNA和双链RNA的活性显示是由一种性质类似于牛胰腺核糖核酸酶A的单一酶引起的。为作比较，测定了鲸、大鼠和牛的结晶核糖核酸酶对双链RNA的活性，发现分别是它们对单链RNA活性的0.4%、0.03%和0.003%。人血清和尿液都含有胰腺来源的核糖核酸酶成分，它们水解双链RNA的速度分别是水解单链RNA速度的2%和0.4%。相比之下，从人脾脏和肝脏中纯化得到的酸稳定核糖核酸酶水解双链RNA的速度至少比人胰腺核糖核酸酶慢20倍，相对于它们对单链RNA的相应速度而言。人胰腺和血清中的酶对双链聚（I）-聚（C）的聚（C）成分表现出明显活性；它们对聚（C）本身的攻击速度约是聚（U）的25倍，是单链RNA的50多倍。
