Kotzé J P
Appl Microbiol. 1969 Nov;18(5):744-7. doi: 10.1128/am.18.5.744-747.1969.
The activities of 15 glycolytic and related enzymes were determined in clostridia. All contained 1-phosphofructokinase; three of them lacked 6-phosphofructokinase and mannitol 1-phosphate dehydrogenase. Glucose 6-phosphate dehydrogenase was found in six clostridia, thus demonstrating the presence of hexose monophosphate shunt. Only parts of the citric acid cycle were found to be present in most clostridia with an indication of the full cycle in Clostridium septicum. The intermediary enzyme activities were used to differentiate between the different clostridia.
对梭菌中的15种糖酵解及相关酶的活性进行了测定。所有梭菌都含有1-磷酸果糖激酶;其中三种缺乏6-磷酸果糖激酶和甘露醇1-磷酸脱氢酶。在六种梭菌中发现了葡萄糖6-磷酸脱氢酶,从而证明了磷酸己糖旁路的存在。在大多数梭菌中仅发现部分柠檬酸循环存在,而在败血梭菌中有完整循环的迹象。利用中间酶活性来区分不同的梭菌。