Orth D S, Anderson A W, Montgomery M W
Appl Microbiol. 1969 Nov;18(5):906-10. doi: 10.1128/am.18.5.906-910.1969.
Egg white trypsin inhibitor activated coagulase clotting when added to a final concentration between 2 and 60 mg/ml. The greatest increase in clotting rate was observed in reaction mixtures containing the lowest concentrations of serum and plasma. Maximal activation was reached with 40 mg of trypsin inhibitor per ml when either serum or plasma was used as the source of coagulase-reacting factor (CRF). The increased rate of clotting is partly due to inhibition of plasmin. Freezing and thawing reduced plasma clotting inhibition. Soybean trypsin inhibitor also activated the coagulase reaction. The increased rate of clotting was observed with a coagulase preparation from organisms which produced plasminogen activators and with the culture supernatant fraction from organisms which did not activate plasminogen to plasmin. The tube test for coagulase could be made more sensitive for some strains of staphylococci by increasing the concentration of CRF (added as plasma or serum) by adding trypsin inhibitor, or both.
当蛋清胰蛋白酶抑制剂添加至终浓度为2至60毫克/毫升时可激活凝固酶凝血反应。在含有最低浓度血清和血浆的反应混合物中观察到凝血速率的最大增加。当使用血清或血浆作为凝固酶反应因子（CRF）的来源时，每毫升40毫克的胰蛋白酶抑制剂可达到最大激活效果。凝血速率增加部分归因于纤溶酶的抑制。冻融降低了血浆凝血抑制作用。大豆胰蛋白酶抑制剂也可激活凝固酶反应。在用产生纤溶酶原激活剂的生物体的凝固酶制剂以及未将纤溶酶原激活为纤溶酶的生物体的培养上清液组分中观察到凝血速率增加。对于某些葡萄球菌菌株，通过添加胰蛋白酶抑制剂或同时添加胰蛋白酶抑制剂和CRF（以血浆或血清形式添加）来提高CRF浓度，可使凝固酶试管试验更灵敏。
