用于凝固酶试验的动物血清适用性比较。
Comparison of animal sera for suitability in coagulase testing.
作者信息
Orth D S, Chugg L R, Anderson A W
出版信息
Appl Microbiol. 1971 Mar;21(3):420-5. doi: 10.1128/am.21.3.420-425.1971.
The sera of several animals were examined for suitability in coagulase testing. The assay for coagulase-reacting factor (CRF) activities of the whole sera indicated the following relative concentrations of CRF: human > pig > rabbit > horse > bovine, chicken, and lamb. Human, pig, and rabbit sera had adequate amounts of CRF for coagulase testing. The plasmin activities of the different sera, arranged from the strongest to the weakest, were as follows: rabbit > human > lamb > horse > bovine, chicken, and pig. Fibrinolysis was observed when rabbit, human, lamb, or horse sera were incorporated into coagulase test agars. Pig serum was superior to the other sera for use in the plate test for coagulase since it had adequate amounts of CRF and the plasminogen-plasmin system was not activated by staphylokinase or staphylococcal Müller factor. Heparinized pig plasma was more suitable than citrated pig plasma since citrate interfered with the growth of Staphylococcus aureus, and the use of heparinized plasma prevented false-positive coagulase reactions due to citrate utilization.
检测了几种动物的血清在凝固酶试验中的适用性。对全血清中凝固酶反应因子（CRF）活性的检测表明，CRF的相对浓度如下：人＞猪＞兔＞马＞牛、鸡和羊。人、猪和兔血清含有足够量的CRF用于凝固酶检测。不同血清的纤溶酶活性从强到弱依次为：兔＞人＞羊＞马＞牛、鸡和猪。当将兔、人、羊或马血清加入凝固酶试验琼脂中时，可观察到纤维蛋白溶解现象。猪血清在凝固酶平板试验中优于其他血清，因为它含有足够量的CRF，且纤溶酶原 - 纤溶酶系统不会被葡萄球菌激酶或葡萄球菌Müller因子激活。肝素化猪血浆比枸橼酸化猪血浆更合适，因为枸橼酸盐会干扰金黄色葡萄球菌的生长，使用肝素化血浆可防止因枸橼酸盐利用导致的假阳性凝固酶反应。
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