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[蟑螂和鲷鱼的鱼卵及精子中的蛋白质及其反应基团]

[Proteins and their reactive groups in the roe and sperm of roach and bream].

作者信息

Konovalov Iu D, Zhukinskiĭ V N

出版信息

Ukr Biokhim Zh (1978). 1979 Jan-Feb;51(1):7-9.

PMID:425142
Abstract

The content of proteins soluble in the phosphate buffer in the mature eggs and sperm of the bream is higher than that in the corresponding cells of the roach. The eggs of both the roach and bream is richer in these proteins than the sperm. The amount of SH- and S-S-groups in proteins of sex cells is not the same and varies depending on the fish species. The ionization coefficient value for lateral groups of proteins soluble in the phosphate buffer at 245 and 300 nm is lower for the eggs of the roach as compared to that of the bream and for the sperm of the roach, vice versa, it is higher than for the bream sperm. This index for proteins of the roach and bream eggs at 245 and 300 nm is higher considerably as compared to that established for sperm.

摘要

鲷成熟卵子和精子中可溶于磷酸盐缓冲液的蛋白质含量高于拟鲤相应细胞中的蛋白质含量。拟鲤和鲷的卵子中这些蛋白质比精子中更丰富。性细胞蛋白质中的SH-和S-S-基团数量不同,且因鱼类种类而异。在245和300nm处,拟鲤卵子中可溶于磷酸盐缓冲液的蛋白质侧链的电离系数值低于鲷卵子,而拟鲤精子的该值则高于鲷精子。与精子相比,拟鲤和鲷卵子蛋白质在245和300nm处的这一指标要高得多。

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