Mankin H J, Lippiello L
J Clin Invest. 1971 Aug;50(8):1712-9. doi: 10.1172/JCI106660.
The cartilages from the hip joints of 13 normal and 15 osteoarthritic humans were analyzed for glycosaminoglycan content and distribution. The GAGs were separated by elution with CPC on a short cellulose column by the technique of Svejcar and Robertson after digestion of the tissue with pronase and papain. The eluates were identified by a variety of methods including determination of molar ratios, N-acetyl-hexosamine determinations after hyaluronidase treatment and thin-layer chromatography of unhydrolyzed and hydrolyzed GAGs. From the data obtained, it was demonstrated that cartilage from arthritic patients showed a significant increase in the concentration of chondroitin 4-sulfate and a significant decrease in keratan sulfate, with only slight changes in the total amount of GAG present. Calculations of the molar ratios showed variation in the sulfation with chondroitin 4-sulfate appearing in the "supersulfated" state in the arthritic cartilage. The data lead to speculation regarding the process of osteoarthritis, and it is concluded that the changes seen are more likely to represent an altered pattern of synthesis rather than selective degradation. Since the changes suggest a younger cartilage, a theory is advanced that the chondrocyte responds to the chronic stress of osteoarthritis by modulation to a chondroblastic phase.
对13名正常人和15名骨关节炎患者髋关节的软骨进行了糖胺聚糖含量和分布分析。在用链霉蛋白酶和木瓜蛋白酶消化组织后，通过Svejcar和Robertson的技术，在短纤维素柱上用十六烷基吡啶氯化物（CPC）洗脱来分离糖胺聚糖。通过多种方法对洗脱液进行鉴定，包括测定摩尔比、透明质酸酶处理后的N-乙酰己糖胺测定以及未水解和水解糖胺聚糖的薄层色谱分析。从获得的数据可以看出，骨关节炎患者的软骨中硫酸软骨素4-硫酸酯浓度显著增加，硫酸角质素显著减少，而糖胺聚糖总量仅有轻微变化。摩尔比计算表明，硫酸软骨素4-硫酸酯在骨关节炎软骨中以“超硫酸化”状态存在，硫酸化存在差异。这些数据引发了对骨关节炎进程的推测，得出的结论是，所观察到的变化更可能代表合成模式的改变而非选择性降解。由于这些变化表明软骨更年轻化，因此提出一种理论，即软骨细胞通过调节进入成软骨细胞阶段来应对骨关节炎的慢性应激。
