硫酸软骨素4和硫酸软骨素蛋白聚糖与胶原蛋白的相互作用性质
Nature of the interaction of chondroitin 4-sulphate and chondroitin sulphate-proteoglycan with collagen.
作者信息
Obrink B, Wasteson A
出版信息
Biochem J. 1971 Jan;121(2):227-33. doi: 10.1042/bj1210227.
Abstract
The electrostatic interaction of chondroitin sulphate and the chondroitin sulphate-proteoglycan with collagen was studied by chromatography of the glycosaminoglycan and the proteoglycan on a collagen gel. The observed binding between the macromolecules increased with decreasing pH and ionic strength, and was significant under physiological conditions. A study of the interaction between chondroitin sulphate and a preparation of soluble collagen, with a partition-equilibrium technique, afforded similar results.
摘要
通过将糖胺聚糖和蛋白聚糖在胶原蛋白凝胶上进行色谱分析，研究了硫酸软骨素以及硫酸软骨素-蛋白聚糖与胶原蛋白之间的静电相互作用。观察到的大分子之间的结合随着pH值和离子强度的降低而增加，并且在生理条件下具有显著性。采用分配平衡技术对硫酸软骨素与可溶性胶原蛋白制剂之间的相互作用进行研究，也得到了类似的结果。
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