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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Dingle J T, Barrett A J, Weston P D

Biochem J. 1971 Jun;123(1):1-13. doi: 10.1042/bj1230001.

Antisera were raised against lysosomal cathepsin D of man, chicken and rabbit. 2. The antisera were found to be specific and potent inhibitors of cathepsin D activity. 3. The immunological nature of the inhibition was established. 4. The inhibitory effect was studied by varying pH, antiserum/enzyme ratio, time of incubation, concentration of components and order of mixing, and by using purified antibody and univalent antibody fragments. 5. The specificities of the antisera were examined with respect to other enzymes, isoenzymes of cathepsin D and cathepsin D from different organs. 6. The antisera prevented the action of cathepsin D on isolated proteoglycans and on cartilage. 7. The antisera produced up to 90% inhibition of the autolysis of cartilage from chicks and rabbits, indicating that cathepsin D is the enzyme mainly responsible for the breakdown of proteoglycans in this system.

制备了针对人、鸡和兔溶酶体组织蛋白酶D的抗血清。2. 发现这些抗血清是组织蛋白酶D活性的特异性强效抑制剂。3. 确定了抑制作用的免疫学性质。4. 通过改变pH值、抗血清/酶比例、孵育时间、各组分浓度和混合顺序，以及使用纯化抗体和单价抗体片段来研究抑制作用。5. 针对其他酶、组织蛋白酶D的同工酶以及来自不同器官的组织蛋白酶D，检测了抗血清的特异性。6. 抗血清可阻止组织蛋白酶D对分离的蛋白聚糖和软骨的作用。7. 抗血清对雏鸡和兔软骨的自溶作用产生高达90%的抑制，表明组织蛋白酶D是该系统中主要负责蛋白聚糖分解的酶。

Dingle J T, Barrett A J, Weston P D

Biochem J. 1971 Jun;123(1):1-13. doi: 10.1042/bj1230001.

Antisera were raised against lysosomal cathepsin D of man, chicken and rabbit. 2. The antisera were found to be specific and potent inhibitors of cathepsin D activity. 3. The immunological nature of the inhibition was established. 4. The inhibitory effect was studied by varying pH, antiserum/enzyme ratio, time of incubation, concentration of components and order of mixing, and by using purified antibody and univalent antibody fragments. 5. The specificities of the antisera were examined with respect to other enzymes, isoenzymes of cathepsin D and cathepsin D from different organs. 6. The antisera prevented the action of cathepsin D on isolated proteoglycans and on cartilage. 7. The antisera produced up to 90% inhibition of the autolysis of cartilage from chicks and rabbits, indicating that cathepsin D is the enzyme mainly responsible for the breakdown of proteoglycans in this system.

制备了针对人、鸡和兔溶酶体组织蛋白酶D的抗血清。2. 发现这些抗血清是组织蛋白酶D活性的特异性强效抑制剂。3. 确定了抑制作用的免疫学性质。4. 通过改变pH值、抗血清/酶比例、孵育时间、各组分浓度和混合顺序，以及使用纯化抗体和单价抗体片段来研究抑制作用。5. 针对其他酶、组织蛋白酶D的同工酶以及来自不同器官的组织蛋白酶D，检测了抗血清的特异性。6. 抗血清可阻止组织蛋白酶D对分离的蛋白聚糖和软骨的作用。7. 抗血清对雏鸡和兔软骨的自溶作用产生高达90%的抑制，表明组织蛋白酶D是该系统中主要负责蛋白聚糖分解的酶。