Rettenmaier R, Vuilleumier J P, Müller-Mulot W
Z Lebensm Unters Forsch. 1979 Feb 26;168(2):120-4. doi: 10.1007/BF01127517.
A fluorometric method for the estimation of the vitamin B1 content in food and biological materials is described. Acid hydrolysis is followed by enzymatic treatment to liberate the vitamin B1 of the sample. Interfering substances in the extract are removed to a great extent by chromatography on Amberlite CG 50, a weakly acidic cation exchanger. After oxidation and extraction into an organic medium, the fluorescence of thiochrome is measured. The procedure is simple, reproducible and not susceptible to interference. The recoveries are good.
描述了一种用于估算食品和生物材料中维生素B1含量的荧光测定方法。先进行酸水解，然后进行酶处理以释放样品中的维生素B1。提取物中的干扰物质通过在弱酸性阳离子交换剂Amberlite CG 50上进行色谱分离，在很大程度上得以去除。经过氧化并萃取到有机介质中后，测定硫色素的荧光。该方法简单、可重复且不易受干扰。回收率良好。
