Coulam C B, Ryan R J
Am J Obstet Gynecol. 1979 Mar 15;133(6):639-43. doi: 10.1016/0002-9378(79)90011-5.
The premature menopause syndrome has been regarded as one of the organ-specific autoimmune disorders because circulating antibodies to ovarian tissue have been demonstrated. Fifteen women with spontaneous cessation of menses after initial menarche before they were 35 years old were seen between 1975 and 1977. Increased serum levels of gonadotropin and failure of estrogen secretion were documented. Serum from each woman was examined for antibodies to normal ovary. Proteins from ovaries of normal premenopausal women were extracted and iodinated with 125I. The labeled proteins were incubated with sera for 48 hours, after which goat antihuman gamma-globulin was added and allowed to incubate for 72 hours. The precipitate was washed and evaluated for radioactive label. The binding of antibodies increased in the sera of patients with the premature menopause syndrome, compared with the control sera. This suggests that the etiology of premature menopause may be mediated by circulating antibodies to ovarian tissue.
早发性绝经综合征被视为器官特异性自身免疫性疾病之一，因为已证实存在针对卵巢组织的循环抗体。1975年至1977年间，共诊治了15名初潮后在35岁之前自然停经的女性。记录到她们血清促性腺激素水平升高且雌激素分泌功能衰竭。检测了每名女性血清中针对正常卵巢的抗体。提取绝经前正常女性卵巢中的蛋白质，并用125I进行碘化。将标记后的蛋白质与血清孵育48小时，之后加入山羊抗人γ球蛋白并再孵育72小时。洗涤沉淀物并评估放射性标记情况。与对照血清相比，早发性绝经综合征患者血清中抗体的结合增加。这表明早发性绝经的病因可能由针对卵巢组织的循环抗体介导。
