Prasad A S, Fernandez-Madrid F, Ryan J R
Am J Physiol. 1979 Mar;236(3):E272-5. doi: 10.1152/ajpendo.1979.236.3.E272.
The activity of deoxythymidine kinase was assayed in implanted sponge connective tissue in three groups of subjects: 1) five normal controls (having normal levels of plasma and red cell zinc); 2) four patients with sickle cell anemia who had low zinc in red cells and hair; and 3) two volunteers (under strict dietary controls), after 6 mo of zinc restriction (2.7 mg/day) and repeated after 3 mo of zinc repletion (30 mg/day). Total protein, total collagen, RNA/DNA, and deoxythymidine kinase activity were measured by techniques reported previously. In sickle cell anemia patients, deoxythymidine kinase activity was not detected, and RNA/DNA, total collagen, and total protein contents were decreased compared to normal controls (statistically significant). In human volunteers deoxythymidine kinase activity was not detected during the zinc restriction phase. After supplementation with zinc, deoxythymidine kinase activity became 70% of normal control levels, and RNA/DNA, total collagen, and total protein contents of sponge connective tissue increased. In conclusion, an adverse effect of zinc deficiency on deoxythymidine kinase activity of implanted sponge connective tissue of man has been demonstrated for the first time.
1）五名正常对照者（血浆和红细胞锌水平正常）；2）四名镰状细胞贫血患者，其红细胞和头发中的锌含量较低；3）两名志愿者（严格饮食控制），在锌限制6个月（2.7毫克/天）后以及锌补充3个月（30毫克/天）后再次进行检测。通过先前报道的技术测量了总蛋白、总胶原蛋白、RNA/DNA和脱氧胸苷激酶活性。在镰状细胞贫血患者中，未检测到脱氧胸苷激酶活性，与正常对照相比，RNA/DNA、总胶原蛋白和总蛋白含量降低（具有统计学意义）。在人类志愿者中，锌限制阶段未检测到脱氧胸苷激酶活性。补充锌后，脱氧胸苷激酶活性达到正常对照水平的70%，海绵结缔组织的RNA/DNA、总胶原蛋白和总蛋白含量增加。总之，首次证明了锌缺乏对人体植入海绵结缔组织脱氧胸苷激酶活性有不良影响。
