Pugh G W, Hughes D E, Schulz V D
Can J Comp Med. 1973 Jan;37(1):70-8.
In three experiments, cattle, mice and guinea pigs were inoculated with viable cultures of Moraxella bovis or fractions of this organism. Fractions were obtained by disruption of cells with a fractionator at 20,000 pounds per square inch, and separating the cell wall and cell sap fractions by differential centrifugation. Cell sap fractions were further separated by ultra-centrifugation, heating and precipitation with (NH(4))(2) SO(4). Different fractions induced different pathophysiological manifestations. The cell wall fractions caused localized lesions (necrosis) at the site of injection, and emphysema and congestion of the lungs. Cell sap fractions induced a "shock syndrome," as well as hemorrhage and inflammation of the intestines, hemorrhage and congestion of lymph nodes, liver, adrenal and spleen. Cell sap also induced conjunctivitis in mice and guinea pigs, and periocular edema, myosis, ocular pruritus and lacrimation in cattle. The authors suggest that M. bovis probably produces endotoxins and exotoxins as well as possibly a specific oculopathic substance, but more definitive work is needed to confirm this. They caution that consideration of these toxins should be made in any application of M. bovis for vaccines or other immunological studies.
在三项实验中，给牛、小鼠和豚鼠接种了牛莫拉菌的活菌培养物或该生物体的组分。通过使用均浆器在每平方英寸20000磅的压力下破碎细胞，并通过差速离心分离细胞壁和细胞液组分来获得这些组分。细胞液组分通过超速离心、加热和用硫酸铵沉淀进一步分离。不同的组分引发了不同的病理生理表现。细胞壁组分在注射部位引起局部病变（坏死），以及肺部气肿和充血。细胞液组分引发了“休克综合征”，以及肠道出血和炎症、淋巴结、肝脏、肾上腺和脾脏出血和充血。细胞液还在小鼠和豚鼠中诱发结膜炎，在牛中诱发眼周水肿、瞳孔缩小、眼部瘙痒和流泪。作者认为，牛莫拉菌可能产生内毒素和外毒素，以及可能一种特定的眼病致病物质，但需要更多确凿的研究来证实这一点。他们警告说，在将牛莫拉菌用于疫苗或其他免疫学研究的任何应用中，都应考虑这些毒素。
