Wardlaw A C, Boorman L, Reid R
Br J Exp Pathol. 1971 Apr;52(2):198-208.
endotoxin injected into CMRL mice in a 23° environment produced a fall in rectal temperature at 3 hr post-injection. Endotoxin given i.v. produced a greater hypothermia than if injected i.p. Pretreatment of the mice with pertussis vaccine, BCG or substantially increased their responsiveness to endotoxin. SJL mice were more sensitive to endotoxin than CMRL mice and showed hypothermias as great as -4·9°. Endotoxins from and had a similar activity to endotoxin, but was less active. There was no evidence to support the idea that endotoxin responsiveness depends on the mice having previous environmental exposure to the corresponding bacterium in the gut flora. The relative endotoxic activities of 1 batch each of pertussis, TAB and cholera vaccines were assayed by the hypothermias produced in SJL mice. Cholera vaccine was twice as potent as TAB which was 4 times more active than pertussis. The index of precision of the mouse hypothermia test (0·32-0·65) is not inferior to that of the standard rabbit pyrogenicity test (about 0·6). The test should be useful for comparing the endotoxic activities of preparations such as bacterial vaccines which may be rich in endotoxin.
在23°环境中给CMRL小鼠注射内毒素，注射后3小时直肠温度下降。静脉注射内毒素比腹腔注射产生的体温过低更明显。用百日咳疫苗、卡介苗对小鼠进行预处理，或 显著增强了它们对内毒素的反应性。SJL小鼠比CMRL小鼠对内毒素更敏感，体温过低可达 -4·9°。来自 和 的内毒素与 内毒素具有相似的活性，但 活性较低。没有证据支持内毒素反应性取决于小鼠之前在肠道菌群中接触过相应细菌 的观点。通过SJL小鼠产生的体温过低来测定1批百日咳疫苗、伤寒副伤寒甲乙三联疫苗和霍乱疫苗的相对内毒素活性。霍乱疫苗的效力是伤寒副伤寒甲乙三联疫苗的两倍，而伤寒副伤寒甲乙三联疫苗的活性是百日咳疫苗的4倍。小鼠体温过低试验的精密度指数（0·32 - 0·65）不低于标准兔热源性试验的精密度指数（约0·6）。该试验对于比较可能富含内毒素的制剂（如细菌疫苗）的内毒素活性应该是有用的。
