Andersson L C, Nordling S, Häyry P
J Exp Med. 1973 Jul 1;138(1):324-9. doi: 10.1084/jem.138.1.324.
Electrophoretically fractionated CBA/Ca spleen T cells alone respond to allogeneic cells in one-way MLC and to PHA. They do not respond to E. coli LPS. B cells alone do not respond to allogeneic cells nor to PHA, but do respond to LPS. When karyotypically distinguishable syngeneic mixtures of T and B lymphocytes are stimulated with allogeneic cells, at the most 5% of mitoses on 5-9th culture day are of B cell origin. This indicates that B cells are not substantially recruited to proliferate in the MLC.
经电泳分离的CBA/Ca脾T细胞单独存在时,能在单向混合淋巴细胞培养中对同种异体细胞作出反应,也能对PHA作出反应。它们对大肠杆菌脂多糖无反应。单独的B细胞对同种异体细胞和PHA均无反应,但对脂多糖有反应。当用同种异体细胞刺激T和B淋巴细胞的核型可区分的同基因混合物时,在培养第5 - 9天,最多5%的有丝分裂细胞起源于B细胞。这表明在混合淋巴细胞培养中,B细胞并没有大量被募集来增殖。