多克隆人T淋巴细胞激活导致人B淋巴细胞的继发性功能激活。
Polyclonal human T lymphocyte activation results in the secondary functional activation of the human B lymphocyte.
作者信息
Bird A G, Hammarström L, Smith C I, Britton S
出版信息
Clin Exp Immunol. 1981 Jan;43(1):165-73.
The polyclonal T lymphocyte activators Con A and PHA were demonstrated to induce secretion of IgM and IgG as well as IgA antibodies in cultures of human peripheral blood lymphocytes. A similar response was seen in one-way mixed lymphocyte cultures and the kinetics, dose-response characteristics and optimal culture conditions are presented. The presence of functional T lymphocytes is a prerequisite for in vitro B lymphocyte activation in response to T lymphocyte mitogens. A narrow dose-response profile is a characteristic for both Con A and PHA and polyclonal B cell activation occurred at what have been previously regarded as suboptimal concentrations of these agents. The use of higher doses of these activators failed to generate immunoglobulin-secreting cells despite the presence of early and optimal levels of DNA synthesis in the cultures.
已证明多克隆T淋巴细胞激活剂刀豆球蛋白A（Con A）和植物血凝素（PHA）可在人外周血淋巴细胞培养物中诱导IgM、IgG以及IgA抗体的分泌。在单向混合淋巴细胞培养中也观察到了类似反应，并给出了其动力学、剂量反应特性和最佳培养条件。功能性T淋巴细胞的存在是体外B淋巴细胞对T淋巴细胞有丝分裂原作出反应而被激活的前提条件。Con A和PHA都具有狭窄的剂量反应曲线，并且在先前被认为是这些试剂的次优浓度下发生了多克隆B细胞激活。尽管培养物中DNA合成处于早期且水平最佳，但使用更高剂量的这些激活剂未能产生分泌免疫球蛋白的细胞。
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