Talbot P
J Reprod Fertil. 1979 Jan;55(1):9-14. doi: 10.1530/jrf.0.0550009.
When fresh epididymal spermatozoa were cold shocked for 10 or 30 min, then warmed to 24 degrees C, sperm motility was normal, but cold shocking ejaculated or capacitated spermatozoa caused a significant decrease in the percentage of motile spermatozoa and, for capacitated spermatozoa, in the rate of motility. The acrosomes of motile fresh epididymal and ejaculated spermatozoa became crenulated after cold shock, and the percentage of spermatozoa with crenulated acrosomes increased with longer periods of cold shock and was higher when spermatozoa were cold shocked in serum than in saline. When epididymal spermatozoa were cold shocked after incubation for 4 h at 37 degrees C, the acrosomes on spermatozoa which had not undergone an acrosome reaction became swollen and elevated instead crenulated. Epididymal spermatozoa which were cold shocked and then incubated for 4 h at 37 degrees C exhibited acrosome reactions and activation of motility, but had reduced fertilizing capacity when tested in vitro. Spermatozoa incubated in serum and cold shocked were able to penetrate zone-free ova even though their tails had been bent through 180 degrees. It is suggested that cold shock decreases the fertilizing capacity of hamster spermatozoa by interfering with the ability of spermatozoa to bind to and/or penetrate the zone pellucida.
当新鲜附睾精子冷休克10或30分钟，然后升温至24摄氏度时，精子活力正常，但冷休克射出的或获能的精子会导致活动精子百分比显著下降，对于获能精子，还会导致活力速率下降。活动的新鲜附睾精子和射出精子的顶体在冷休克后会出现皱缩，顶体皱缩的精子百分比会随着冷休克时间延长而增加，并且当精子在血清中冷休克时比在盐水中更高。当附睾精子在37摄氏度孵育4小时后冷休克时，未发生顶体反应的精子顶体会肿胀并隆起而非皱缩。冷休克后在37摄氏度孵育4小时的附睾精子会出现顶体反应并激活活力，但在体外测试时受精能力降低。在血清中孵育并冷休克的精子即使其尾部弯曲了180度也能够穿透无透明带的卵子。有人提出，冷休克通过干扰精子与透明带结合和/或穿透透明带的能力来降低仓鼠精子的受精能力。
