Sato K, Dobson R L
J Clin Invest. 1973 Sep;52(9):2166-74. doi: 10.1172/JCI107401.
This paper attempts to further clarify the characteristics of Mecholyl- or epinephrine-stimulated glucose metabolism in the isolated monkey eccrine sweat gland with special emphasis on its relationship to increased sodium transport. The Mecholyl- or epinephrine-stimulated glucose metabolism (as estimated by either lactate or (14)CO(2) production or both) is seen only in the secretory coil and not in the duct. It is markedly suppressed in the absence of glucose, Na(+), or K(+). It is inhibited by ouabain (10(-3) M) and partially suppressed in a low-sodium (40 mM), high-potassium (100 mM) medium.2,4-dinitrophenol (10(-4) M) reverses ouabain-induced inhibition of lactate and (14)CO(2) production but only partially reverses inhibition induced by Na(+) + K(+) deprivation, indicating that metabolic inhibition by ouabain is secondary to the inhibition of sodium transport. There is no synergism between Mecholyl and epinephrine. The absence of any significant inhibitory effects by acetazolamide (Diamox) or HCO(3) (-)-free media suggests that H(+) transport may not be important in sweat gland function. In contrast to a report by Wolfe et al., human eccrine sweat glands show considerable oxidative activity ((14)CO(2) production of 0.42-0.72 nmol/gland/h). These observations are discussed in terms of the linkage between sweat gland energy metabolism and sodium transport.
本文试图进一步阐明在离体猴小汗腺中，乙酰甲胆碱或肾上腺素刺激的葡萄糖代谢的特征，特别强调其与钠转运增加的关系。乙酰甲胆碱或肾上腺素刺激的葡萄糖代谢（通过乳酸或（14）CO2产生或两者来估计）仅在分泌蟠管中可见，而在导管中则不可见。在没有葡萄糖、Na+或K+的情况下，其会被显著抑制。它会被哇巴因（10（-3）M）抑制，并且在低钠（40 mM）、高钾（100 mM）培养基中会被部分抑制。2,4-二硝基苯酚（10（-4）M）可逆转哇巴因诱导的乳酸和（14）CO2产生的抑制，但仅部分逆转由Na+ + K+缺乏诱导的抑制，这表明哇巴因引起的代谢抑制是钠转运抑制的继发效应。乙酰甲胆碱和肾上腺素之间没有协同作用。乙酰唑胺（醋氮酰胺）或无HCO（3）（-）培养基没有任何显著抑制作用，这表明H+转运在汗腺功能中可能并不重要。与Wolfe等人的报告相反，人小汗腺显示出相当高的氧化活性（（14）CO2产生为0.42 - 0.72 nmol/腺体/小时）。本文根据汗腺能量代谢与钠转运之间的联系对这些观察结果进行了讨论。
