Department of Biology, San Diego State University, 5500 Campanile Drive, San Diego, CA 92182-7251, USA.
J Physiol Sci. 2010 Mar;60(2):103-7. doi: 10.1007/s12576-009-0073-3. Epub 2009 Dec 16.
The purpose of this study was to determine the effect of increases in exercise intensity on the sweat lactate concentration and lactate excretion rate. Eight healthy male volunteers complete a 90-min exercise bout of treadmill walking in a 35 degrees C and 40% relative humidity environmental chamber. During the exercise trial, the subjects performed three 30-min ordered exercise bouts at 60, 70, and 80% of their age-predicted maximum heart rate (HR(max)), with 10 min of rest outside the chamber between bouts. Sweat rate was measured volumetrically during each of the three exercise bouts on the flexor surface of the proximal half of the right forearm. Sweat lactate concentration (lactate) was measured in each sample and multiplied by the forearm sweat rate to calculate the lactate excretion rate (LER). There was a significant (P < 0.05) decrease in the lactate at the 70 and 80% HR(max) exercise intensities compared to the 60% HR(max) exercise intensity. Conversely, the LER increased significantly at the highest two exercise intensities compared to the 60% HR(max) exercise intensity. Such data suggest that increases in exercise intensity require an increase in lactate production, as measured by the LER. Furthermore, the decreased lactate at the higher exercise intensities is most likely the result of increased sweat production causing a dilution effect on the lactate, thus limiting its ability to accurately indicate the metabolic activity of the sweat gland.
本研究旨在确定运动强度增加对汗乳酸浓度和乳酸排泄率的影响。8 名健康男性志愿者在 35°C 和 40%相对湿度的环境室中完成 90 分钟的跑步机行走运动。在运动试验中,受试者以 60%、70%和 80%的年龄预测最大心率(HR(max))进行了三次 30 分钟的有序运动,每次运动之间在室外交替休息 10 分钟。在三个运动期间,通过测量右前臂近端半部分屈肌表面的体积来测量汗率。测量每个样本中的汗乳酸浓度(lactate),并将其乘以前臂汗率以计算乳酸排泄率(LER)。与 60%HR(max)运动强度相比,70%和 80%HR(max)运动强度时Lactate显著降低(P < 0.05)。相反,与 60%HR(max)运动强度相比,最高两个运动强度时 LER 显著增加。这些数据表明,与 60%HR(max)运动强度相比,运动强度的增加需要增加乳酸的产生,这可以通过 LER 来衡量。此外,在较高运动强度下Lactate降低的原因很可能是汗液分泌增加导致Lactate稀释,从而限制了其准确指示汗腺代谢活动的能力。