Resonance Raman study of the dark-adapted form of the purple membrane protein.
作者信息
Aton B, Doukas A G, Callender R H, Becher B, Ebrey T G
出版信息
Biochim Biophys Acta. 1979 Feb 26;576(2):424-8. doi: 10.1016/0005-2795(79)90417-3.
PMID:427199
Abstract
The resonance Raman spectrum of the dark-adapted form of the purple membrane protein (bacteriorhodopsin) has been obtained and is compared to the light-adapted pigment and model chromophore spectra. As in the light-adapted form, the chromophore-protein linkage is found to be a protonated Schiff base. Electron delocalization appears to play the dominant role in color regulation. The dark-adapted spectrum indicates a conformation closer to 13-cis than the light-adapted spectrum.
摘要
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