高压对细菌视紫红质吸收光谱和异构体组成的影响。
Effect of high pressure on the absorption spectrum and isomeric composition of bacteriorhodopsin.
作者信息
Tsuda M, Ebrey T G
出版信息
Biophys J. 1980 Apr;30(1):149-57. doi: 10.1016/S0006-3495(80)85083-1.
Abstract
The effects of high pressure upon the absorption spectra and isomeric composition of the dark (bRD) and light adapted (bRL) forms of bacteriorhodopsin were examined. Pressure favors the 13-cis form of bacteriorhodopsin (bR13-cis). The equilibrium isomeric composition and absorption spectra of bacteriorhodopsin samples at a given pressure were the same starting from either light or dark adapted bacteriorhodopsin. From the effect of pressure on the equilibrium constant between bRall-trans in equilibrium bR13-cis in the dark, the molar volume change between bRall-trans and bR13-cis was found to be -7.8 +/- 3.2 ml/mol. This volume change suggests a difference in conformation between dark- and light-adapted bacteriorhodopsin, but the magnitude of the change is small, involving only a small number of the protein residues.
摘要
研究了高压对细菌视紫红质暗适应形式（bRD）和光适应形式（bRL）的吸收光谱及异构体组成的影响。压力有利于细菌视紫红质的13-顺式形式（bR13-cis）。在给定压力下，无论起始于光适应还是暗适应的细菌视紫红质，其平衡异构体组成和吸收光谱都是相同的。根据压力对暗处bR全反式与bR13-顺式平衡常数的影响，发现bR全反式与bR13-顺式之间的摩尔体积变化为-7.8±3.2 ml/mol。这种体积变化表明暗适应和光适应的细菌视紫红质在构象上存在差异，但变化幅度较小，仅涉及少数蛋白质残基。
相似文献
1
Effect of high pressure on the absorption spectrum and isomeric composition of bacteriorhodopsin.高压对细菌视紫红质吸收光谱和异构体组成的影响。
Biophys J. 1980 Apr;30(1):149-57. doi: 10.1016/S0006-3495(80)85083-1.
2
Isomeric composition of retinal chromophore in dark-adapted bacteriorhodopsin.暗适应细菌视紫红质中视黄醛发色团的异构体组成。
J Biochem. 1977 Dec;82(6):1599-604. doi: 10.1093/oxfordjournals.jbchem.a131855.
3
Two processes lead to a stable all-trans and 13-cis isomer equilibrium in dark-adapted bacteriorhodopsin; effect of high pressure on bacteriorhodopsin, bacteriorhodopsin mutant D96N and fluoro-bacteriorhodopsin analogues.在暗适应的细菌视紫红质中，两个过程导致稳定的全反式和顺-13-视黄醛异构体平衡；高压对细菌视紫红质、细菌视紫红质突变体D96N和氟细菌视紫红质类似物的影响。
Eur Biophys J. 2002 Dec;31(7):539-48. doi: 10.1007/s00249-002-0250-2. Epub 2002 Sep 26.
4
On the photocycle and light adaptation of dark-adapted bacteriorhodopsin.关于暗适应细菌视紫红质的光循环和光适应
Biophys J. 1977 Aug;19(2):185-9. doi: 10.1016/S0006-3495(77)85579-3.
5
High-pressure near-infrared Raman spectroscopy of bacteriorhodopsin light to dark adaptation.细菌视紫红质从光适应到暗适应的高压近红外拉曼光谱。
Biophys J. 1995 Oct;69(4):1554-62. doi: 10.1016/S0006-3495(95)80027-5.
6
Formation of 9-cis- and 11-cis-retinal pigments from bacteriorhodopsin by irradiating purple membrane in acid.通过在酸性条件下照射紫膜，由细菌视紫红质形成9-顺式和11-顺式视黄醛色素。
Biochemistry. 1980 Aug 5;19(16):3825-31. doi: 10.1021/bi00557a027.
7
Photoconversion from the light-adapted to the dark-adapted state of bacteriorhodopsin.细菌视紫红质从光适应状态到暗适应状态的光转换。
Biophys J. 1985 Aug;48(2):201-8. doi: 10.1016/S0006-3495(85)83773-5.
8
Effect of light-adaptation on the photoreaction of bacteriorhodopsin from Halobacterium halobium.光适应对嗜盐栖热菌视紫红质光反应的影响。
Biochim Biophys Acta. 1977 Dec 23;462(3):575-82. doi: 10.1016/0005-2728(77)90102-5.
9
Met-145 is a key residue in the dark adaptation of bacteriorhodopsin homologs.甲硫氨酸-145是细菌视紫红质同源物暗适应过程中的关键残基。
Biophys J. 1994 Sep;67(3):1187-91. doi: 10.1016/S0006-3495(94)80587-9.
10
Resonance Raman study of the dark-adapted form of the purple membrane protein.
Biochim Biophys Acta. 1979 Feb 26;576(2):424-8. doi: 10.1016/0005-2795(79)90417-3.
引用本文的文献
1
Dynamic Coupling of Tyrosine 185 with the Bacteriorhodopsin Photocycle, as Revealed by Chemical Shifts, Assisted AF-QM/MM Calculations and Molecular Dynamic Simulations.化学位移辅助的 AF-QM/MM 计算和分子动力学模拟揭示视紫红质光循环中酪氨酸 185 的动态偶联。
Int J Mol Sci. 2021 Dec 18;22(24):13587. doi: 10.3390/ijms222413587.
2
Pressure effects on the dark-adaptation of bacteriorhodopsin.压力对菌紫质暗适应的影响。
Biophys J. 1993 Apr;64(4):1187-93. doi: 10.1016/S0006-3495(93)81484-X.
3
Surface charge movements of purple membrane during light-dark adaptation.紫膜在光暗适应过程中的表面电荷运动。
Biophys J. 1986 Aug;50(2):205-11. doi: 10.1016/S0006-3495(86)83454-3.
4
Pressure dependence of the photocycle kinetics of bacteriorhodopsin.细菌视紫红质光循环动力学的压力依赖性
Biophys J. 2002 Dec;83(6):3490-8. doi: 10.1016/S0006-3495(02)75348-4.
5
Energetics and volume changes of the intermediates in the photolysis of octopus rhodopsin at a physiological temperature.生理温度下章鱼视紫红质光解过程中中间体的能量学和体积变化
Biophys J. 2002 Aug;83(2):1136-46. doi: 10.1016/S0006-3495(02)75237-5.
6
The effect of protein conformation change from alpha(II) to alpha(I) on the bacteriorhodopsin photocycle.蛋白质构象从α(II)转变为α(I)对细菌视紫红质光循环的影响。
Biophys J. 2000 Apr;78(4):2031-6. doi: 10.1016/S0006-3495(00)76750-6.
7
High-pressure near-infrared Raman spectroscopy of bacteriorhodopsin light to dark adaptation.细菌视紫红质从光适应到暗适应的高压近红外拉曼光谱。
Biophys J. 1995 Oct;69(4):1554-62. doi: 10.1016/S0006-3495(95)80027-5.
8
Photoinduced volume changes associated with the early transformations of bacteriorhodopsin: a laser-induced optoacoustic spectroscopy study.与细菌视紫红质早期转变相关的光致体积变化：一项激光诱导光声光谱研究
Biophys J. 1994 Mar;66(3 Pt 1):838-43. doi: 10.1016/s0006-3495(94)80860-4.
9
Fourier transform infrared difference spectroscopy of bacteriorhodopsin and its photoproducts.细菌视紫红质及其光产物的傅里叶变换红外差光谱
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4972-6. doi: 10.1073/pnas.79.16.4972.
10
Effects of pressure and temperature on the M412 intermediate of the bacteriorhodopsin photocycle. Implications for the phase transition of the purple membrane.压力和温度对细菌视紫红质光循环中M412中间体的影响。对紫膜相变的启示。
Biophys J. 1983 Nov;44(2):249-54. doi: 10.1016/S0006-3495(83)84296-9.
本文引用的文献
1
Effects of detergents and high pressures upon the metarhodopsin I--metarhodopsin II equilibrium.去污剂和高压对视紫红质I-视紫红质II平衡的影响。
Biochemistry. 1974 Feb 12;13(4):738-45. doi: 10.1021/bi00701a016.
2
Effect of pressure on biopolymers and model systems.压力对生物聚合物及模型系统的影响。
Symp Soc Exp Biol. 1972;26:103-24.
3
Functions of a new photoreceptor membrane.一种新型光感受器膜的功能。
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2853-7. doi: 10.1073/pnas.70.10.2853.
4
Improved isolation procedures for the purple membrane of Halobacterium halobium.嗜盐菌紫膜的改进分离程序。
Prep Biochem. 1975;5(2):161-78. doi: 10.1080/00327487508061568.
5
Photochemical reaction of bacteriorhodopsin.细菌视紫红质的光化学反应。
FEBS Lett. 1976 Dec 15;72(1):33-8. doi: 10.1016/0014-5793(76)80807-1.
6
Effects of light adaptation on the purple membrane structure of Halobacterium halobium.光适应对嗜盐栖热菌紫膜结构的影响。
Biophys J. 1976 Oct;16(10):1183-200. doi: 10.1016/S0006-3495(76)85767-0.
7
Light-induced formation of the 410 nm intermediate from reconstituted bacteriorhodopsin.
J Biochem. 1977 Oct;82(4):1177-80. doi: 10.1093/oxfordjournals.jbchem.a131792.
8
Identification of retinal isomers isolated from bacteriorhodopsin.从细菌视紫红质中分离出的视网膜异构体的鉴定。
Biochemistry. 1977 May 3;16(9):1955-9. doi: 10.1021/bi00628a031.
9
Isomeric composition of retinal chromophore in dark-adapted bacteriorhodopsin.暗适应细菌视紫红质中视黄醛发色团的异构体组成。
J Biochem. 1977 Dec;82(6):1599-604. doi: 10.1093/oxfordjournals.jbchem.a131855.
10
Resonance Raman study of the dark-adapted form of the purple membrane protein.
Biochim Biophys Acta. 1979 Feb 26;576(2):424-8. doi: 10.1016/0005-2795(79)90417-3.
Zotero 插件