Svasti J, Talupphet N
Biochim Biophys Acta. 1979 Mar 27;577(1):221-5. doi: 10.1016/0005-2795(79)90024-2.
By use of the neutral alkylating agent iodo [14C1] acetamide instead of ethylene imine or iodoacetate, the resolution of human protamines on gel electrophoresis and ion-exchange chromatography has been improved. Using 20-cm gels, human protamines may be fractionated into seven bands, including the two chromatographically distinct forms of HP1 and a hitherto undetected component HP4. On ion-exchange chromatography, HP2 and the two forms of HP1 may be isolated in sufficient purity for sequence analysis.
通过使用中性烷基化剂碘代[¹⁴C₁]乙酰胺替代乙撑亚胺或碘乙酸盐，人精蛋白在凝胶电泳和离子交换色谱上的分离效果得到了改善。使用20厘米的凝胶，人精蛋白可被分离成七条带，包括两种在色谱上不同的HP1形式和一种迄今未检测到的组分HP4。在离子交换色谱上，HP2和两种形式的HP1可以被分离到足够的纯度用于序列分析。
