Nöll G, Stieve H, Winterhager J
Biophys Struct Mech. 1979 Mar 21;5(1):43-53. doi: 10.1007/BF00535772.
The calcium content of bovine rod outer segment (ROS) suspensions was determined by flame spectrophotometry to be about 0.2 Ca2+ per molecule rhodopsin. After bleaching of rhodopsin, a release of 0.01--0.1 Ca2+ per molecule rhodopsin from ROS into the solution was observed. These figures agree with some data in the literature (Appendix). A measured absorption increase of the Ca2+-indicator phthalein purple (10 degrees C, 562 nm, pH 9.3) occurs apparently simultaneously with the formation of metarhodopsin ii in ROS. This indicates that a light induced Ca2+-release of 12 calcium ions per photoactivated rhodopsin is coupled in time with the formation of metarhodopsin II.
通过火焰分光光度法测定，牛视杆外段（ROS）悬浮液中的钙含量约为每分子视紫红质含0.2个Ca2+。视紫红质漂白后，观察到每分子视紫红质有0.01--0.1个Ca2+从ROS释放到溶液中。这些数据与文献中的一些数据（附录）相符。在ROS中，Ca2+指示剂邻苯二甲酚酞紫（10摄氏度，562纳米，pH 9.3）的吸光度增加显然与视紫红质II的形成同时发生。这表明每一个光活化视紫红质光诱导释放12个钙离子的过程在时间上与视紫红质II的形成相关联。
