Abbott J, Schiltz J, Dienstman S, Holtzer H
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1506-10. doi: 10.1073/pnas.71.4.1506.
A single cell isolated from cultured 8-day leg muscle may, when subcultured, yield a myogenic clone. A myogenic clone consists of myotubes and mononucleated cells. When such a myogenic clone is subcultured, large numbers of mononucleated cells are recovered. These mononucleated cells histologically and biochemically are indistinguishable from authentic fibroblasts cultured under the same conditions: they synthesize (alpha1)(2)alpha2 chains of collagen, large amounts of hyaluronic acid, and modest amounts of chondroitin sulfate. These mononucleated cells, however, will not chondrify when grown under culture conditions known to permit presumptive chondroblasts to differentiate terminally. These findings demonstrate that there is a population of single cells in 8-day muscle that is neither a myoblast nor a fibroblast, but is the common progenitor for cells in the myogenic and fibrogenic lineages: this progenitor, however, is beyond the point of readily yielding chondrogenic cells. These findings are discussed in terms of the limited number of phenotypic options open to differentiating cells in each of the successive compartments of their respective lineages.
从培养8天的腿部肌肉中分离出的单个细胞，在传代培养时可能产生一个肌源性克隆。一个肌源性克隆由肌管和单核细胞组成。当这样的肌源性克隆传代培养时，会回收大量的单核细胞。这些单核细胞在组织学和生物化学上与在相同条件下培养的真正成纤维细胞没有区别：它们合成胶原蛋白的（α1）（2）α2链、大量透明质酸和适量硫酸软骨素。然而，这些单核细胞在已知能使假定的成软骨细胞终末分化的培养条件下生长时不会软骨化。这些发现表明，8天龄肌肉中存在一群单细胞，它们既不是成肌细胞也不是成纤维细胞，而是肌源性和纤维源性谱系细胞的共同祖细胞：然而，这个祖细胞已不再容易产生软骨形成细胞。根据分化细胞在其各自谱系的每个连续区室中可选择的有限表型选项对这些发现进行了讨论。
