Zucker W H, Shermer R W, MAson R G
Am J Pathol. 1974 Nov;77(2):255-67.
Ultrastructural features of human platelets separated from blood by a number of different procedures were compared. Platelet isolation procedures included differential centrifugation, gel filtration and albumin density gradient centrifugation. Platelets separated from blood by the differential centrifugation procedure of Mustard showed the least ultrastructural change compared to platelets in plasma. Isolation of platelets from blood by gel filtration produced minor ultrastructural changes including irregularity of cytoplasmic outline, formation of a few short pseudopods and slight dilatation of the surface-connected canalicular system. Platelets separated by albumin density gradient centrifugation showed the most marked alterations of ultrastructure. Ultrastructural features of platelets isolated by the various procedures are compared to functional changes previously reported.
对通过多种不同方法从血液中分离出的人血小板的超微结构特征进行了比较。血小板分离方法包括差速离心、凝胶过滤和白蛋白密度梯度离心。与血浆中的血小板相比，通过Mustard的差速离心法从血液中分离出的血小板超微结构变化最小。通过凝胶过滤从血液中分离血小板会产生轻微的超微结构变化，包括细胞质轮廓不规则、形成一些短伪足以及表面连接的小管系统略有扩张。通过白蛋白密度梯度离心分离的血小板显示出最明显的超微结构改变。将通过各种方法分离的血小板的超微结构特征与先前报道的功能变化进行了比较。
