Weisbrode S E, Capen C C, Nagode L A
Am J Pathol. 1974 Aug;76(2):359-76.
Pharmacologic doses of vitamin D given to uremic rats significantly elevated serum calcium levels and produced dramatic ultrastructural changes in osteoblasts and osteocytes compared to placebo-treated uremic rats. Osteocytes in uremic rats given vitamin D were interpreted to have increased metabolic activity, with greater amounts of rough endoplasmic reticulum, and they contained finely granular mineral in pericellular sheaths. Osteoblasts appeared to have reduced activity with lesser amounts of rough endoplasmic reticulum, increased ribosomes and amorphous mineral frequently deposited along plasma membranes compared to uremic rats given placebo treatment. Osteoclasts were seen more frequently in uremic rats given vitamin D. Bone cell response in sham-operated rats administered vitamin D was distinctly different. Osteocytes and osteoblasts appeared to have greater metabolic activity compared to vitamin D-treated uremic rats. Osteocytes in sham-operated rats had increased amounts of rough endoplasmic reticulum and lysosomes. Osteoblasts were larger, with moderate amounts of rough endoplasmic reticulum, and had no mineral deposits along the plasma membranes. Osteoclasts were more numerous in sham-operated rats administered vitamin D. The altered response of bone cells in uremic rats given vitamin D was postulated to be due to the elevated serum phosphorus and greater release of calcitonin from thyroid C cells.
与给予安慰剂治疗的尿毒症大鼠相比，给予尿毒症大鼠药理剂量的维生素D可显著提高血清钙水平，并使成骨细胞和骨细胞产生显著的超微结构变化。给予维生素D的尿毒症大鼠的骨细胞被认为代谢活性增强，粗面内质网增多，其细胞周鞘内含有细颗粒状矿物质。与给予安慰剂治疗的尿毒症大鼠相比，给予维生素D的尿毒症大鼠的成骨细胞活性似乎降低，粗面内质网减少，核糖体增多，且无定形矿物质经常沿质膜沉积。在给予维生素D的尿毒症大鼠中，破骨细胞更为常见。给予维生素D的假手术大鼠的骨细胞反应明显不同。与给予维生素D治疗的尿毒症大鼠相比，假手术大鼠的骨细胞和成骨细胞似乎具有更高的代谢活性。假手术大鼠的骨细胞粗面内质网和溶酶体数量增加。成骨细胞较大，粗面内质网含量适中，质膜上无矿物质沉积。给予维生素D的假手术大鼠中的破骨细胞更多。给予维生素D的尿毒症大鼠骨细胞反应的改变被推测是由于血清磷升高以及甲状腺C细胞降钙素释放增加所致。
