IgG上的效应器激活决定簇。II. 通过纤溶酶消化兔IgG区分C1q结合位点与细胞毒性K细胞和中性粒细胞的结合位点。
Effector activating determinants on IgG. II. Differentiation of the combining sites for C1q from those for cytotoxic K cells and neutrophils by plasmin digestion of rabbits IgG.
作者信息
MacLennan I C, Connell G E, Gotch F M
出版信息
Immunology. 1974 Feb;26(2):303-10.
Abstract
Plasmin digestion of rabbit IgG after pretreatment at low pH yields a fragment (Facb) which has lost the C3 domain. A study of the biological activity of this fragment is described and the following conclusions are drawn: (1) Facb possesses the same antigen-combining capacity as the whole antibody; (2) C1 fixation is normal; (3) The capacity to sensitize target cells to lysis by cytotoxic K cells is lost; (4) Facb—antigen complexes are unable competitively to inhibit phagocytosis of sensitized bacteria by neutrophils.
摘要
在低pH值下预处理后，兔免疫球蛋白G（IgG）经纤溶酶消化产生一个缺失C3结构域的片段（Facb）。本文描述了对该片段生物活性的研究，并得出以下结论：（1）Facb具有与完整抗体相同的抗原结合能力；（2）C1固定正常；（3）使靶细胞对细胞毒性K细胞介导的裂解敏感的能力丧失；（4）Facb-抗原复合物不能竞争性抑制中性粒细胞对致敏细菌的吞噬作用。
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本文引用的文献
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The hydrolysis of rabbit y-globulin and antibodies with crystalline papain.用结晶木瓜蛋白酶对兔γ球蛋白和抗体进行水解。
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A spectrophotometric determination of trypsin and chymotrypsin.胰蛋白酶和胰凝乳蛋白酶的分光光度测定法
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Complement fixation by antibody fragments.抗体片段介导的补体固定
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Inheritance of haptoglobin subtypes.触珠蛋白亚型的遗传。
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A quantitative immunochemical measure of the primary interaction between I BSA and antibody.一种对牛血清白蛋白（BSA）与抗体之间主要相互作用的定量免疫化学测定方法。
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Complement fixation by the F(ab')2-fragment of pepsin-treated rabbit antibody.经胃蛋白酶处理的兔抗体的F(ab')2片段的补体结合
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Stepwise cleavage of rabbit immunoglobulin G by papain and isolation of four types of biologically active Fc fragments.木瓜蛋白酶对兔免疫球蛋白G的逐步裂解及四种生物活性Fc片段的分离
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