豚鼠腹膜多形核白细胞质膜的Fc受体活性。
An Fc-receptor activity of plasma membranes from guinea-pig peritoneal polymorphonuclear leucocytes.
作者信息
Tsunawaki S, Mizuno D, Kakinuma K, Kasahara M
出版信息
Biochem J. 1982 Jun 15;204(3):625-34. doi: 10.1042/bj2040625.
Plasma membranes prepared from guinea-pig peritoneal polymorphonuclear leucocytes showed an immune-complex-binding activity that corresponded well with the activity in intact cells. The characteristics of this activity were reversible binding, dependence on the Fc portion of antigen-complexed IgG (immunoglobulin G), competition with aggregated IgG and independence from energy metabolism. These results support the conclusion that the binding activity found in the isolated plasma membranes is an Fc-receptor activity of guinea-pig peritoneal polymorphonuclear leucocytes. The activity showed Kd = 6.7 x 10(-8) M-IgG and maximum binding of 17 pmol of IgG/mg of membrane protein when measured with an immune complex of alpha-amylase and homologous guinea-pig anti-(alpha-amylase) IgG. Inhibition of the Fc-receptor activity by a series of various salts indicated the contribution of the hydrophobic interaction to the binding. Inhibitory effects of salts or metal-chelating reagents on the Fc-receptor activity were also observed on superoxide generation by these cells induced by the immune complex, suggesting a role of the Fc receptor as the immune-complex-binding site responsible for the initiation of superoxide generation.
从豚鼠腹膜多形核白细胞制备的质膜显示出一种免疫复合物结合活性，该活性与完整细胞中的活性非常吻合。这种活性的特征是可逆结合、依赖于抗原复合IgG（免疫球蛋白G）的Fc部分、与聚集IgG竞争以及不依赖能量代谢。这些结果支持以下结论：在分离的质膜中发现的结合活性是豚鼠腹膜多形核白细胞的Fc受体活性。当用α-淀粉酶和同源豚鼠抗（α-淀粉酶）IgG的免疫复合物测量时，该活性显示Kd = 6.7×10（-8）M-IgG，最大结合量为17 pmol IgG/mg膜蛋白。一系列不同盐对Fc受体活性的抑制表明疏水相互作用对结合的贡献。在这些细胞由免疫复合物诱导产生超氧化物的过程中，也观察到盐或金属螯合剂对Fc受体活性的抑制作用，这表明Fc受体作为负责启动超氧化物产生的免疫复合物结合位点的作用。
Zotero 插件