血浆纤维蛋白原快速测定方法的评估
Evaluation of a rapid method of determination of plasma fibrinogen.
作者信息
Thomson G W, McSherry B J, Valli V E
出版信息
Can J Comp Med. 1974 Jul;38(3):266-70.
An evaluation was made of a rapid semiautomated method of determining fibrinogen levels in bovine plasma. This method, the fibrometer method of Morse, Panek and Menga (8), is based on the principle that when thrombin is added to suitably diluted plasma the time of clotting is linearly related to the fibrinogen concentration. A standard curve prepared using bovine plasma had an r value of .9987 and analysis of variance showed there was no significant deviation from regression. A comparison of the fibrometer method and the biuret method of Ware, Guest and Seegers done on 158 bovine plasma samples showed good correlation between the two methods. It was concluded that the fibrometer method does measure bovine fibrinogen and has considerable merit for use in clinical diseases of cattle.
对一种测定牛血浆中纤维蛋白原水平的快速半自动方法进行了评估。这种方法，即莫尔斯、帕内克和蒙加（8）的纤维蛋白原测定仪法，基于这样一个原理：当将凝血酶添加到适当稀释的血浆中时，凝血时间与纤维蛋白原浓度呈线性关系。使用牛血浆制备的标准曲线的r值为0.9987，方差分析表明回归无显著偏差。对158份牛血浆样本进行的纤维蛋白原测定仪法和韦尔、格斯特和西格尔斯的双缩脲法的比较显示，这两种方法之间具有良好的相关性。得出的结论是，纤维蛋白原测定仪法确实能测定牛纤维蛋白原，并且在牛的临床疾病中使用具有相当大的优点。
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